Hello experts!! I have 6460 LCMSMS system. It unfortunately failed in tuning even in brand new tuning solution. Auto tune repeatedly failed in peak width adjustment in MS1. What could be the problems and possible answers? Cleaning is done up to ion transfer…
Thanks for the response Matthais. The issue actually is that autotune sometimes fails to match EPA compliance criteria, and so it becomes necessary to manually tweak settings. I get that Agilent doesn't want lab techs carelessly messing around with quadrupole…
Hi Carol_Ultivo , There is always a trade-off or balance to be struck between Scan speed, mass resolution and sensitivity in any quadrupole mass filter. So, if you decrease the resolution and keep scan speeds the same you will naturally get an increase…
I found the issue. I have "Hide duplicates" selected which prevents any additional Fails from being displayed. However, if I uncheck that box, there is a Fail in every row. This is a summary result, is there a way to make it display only once per group…
Hello,
My laboratory runs a multilevel calibration with internal standards on a headspace-GC-FID. Our sequence is set up to replace each level of the curve after each calibrator is analyzed and then calculate the concentration of the analyte with the…
Your second sample set did not start because there is a conflict that could not be resolved. Instrument failures can be separated into method or sequence.
If you select your (failing) instrument method and press "Setup", does the instrument start equilibrating…
The basic tools I would recommend to have for GC maintenance:
Ceramic column cutter
1/4" wrench (column nuts)
7/16" wrench (gas fittings at back of GC)
1/2" wrench (replace gold seal on S/SL inlet)
Torx 20 driver (removing FID collector assembly…
Hi Dr. Know
Here is some of the information you have requested. Please let me know if you need any further info.
How is your system configured? FID detector using helium as carrier gas, nitrogen as makeup gas.
Which phases are the two columns…
Hello Kang: Generally, and aging lamp would be the likely cause of this problem. The average life of the 8453 lamp is 1000 hours. Lamp performance and lifetime may also be affected by physical shock while the lamp is hot, igniting the lamp while it's…
This Information Applies To: Agilent ICP Expert software.
Issue
The software version is often asked and is a valuable information: to see if any updates are needed, if certain options are available etc.
Steps to Follow
Launch ICP Expert...
This Information Applies To: Agilent OpenLab CDS
Issue
This article provides step-by-step instructions to calculate peak-to-valley ratio in OpenLab CDS.
Background
The peak-to-valley ratio is calculated to indicate the quality of peak separation...
This Information Applies To: Agilent 6500 Series Q-TOF LC/MS systems, MassHunter Workstation 10.1 or below and MassHunter Workstation 11.0 .
Issue
Fully reset the Q-TOF LC/MS system via a power cycle without venting. For users who experience slow...
This Information Applies To: Agilent 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer, and Oligo Pro II systems
Issue
The volume of sample used by Agilent parallel capillary electrophoresis systems during a run.
Resolution
The...
This Information Applies To: Agilent 2100 Expert software, 2100 Expert Security Pack, 2100 Bioanalyzer
Issue
When opening the 2100 Expert software and using the Security Pack, the error message " Start of launcher application failed - The requested...
This Information Applies To: Agilent 6890, 6850, 7890, 8890, 8860, Intuvo 9000 GC systems.
Issue
The exchange of the ignitor in a Flame Photometric Detector (FPD) may be required in the following scenarios:
Preventive or periodic maintenance...
This Information Applies To: Agilent 1290 Infinity Quaternary Pump G4204A, 1290 Infinity II Flexible Pumps G7104A/C, and G7131A/C.
Issue
If the flexible/quaternary pump does not generate flow or build up pressure, it could be due to a stuck inlet...
This Information Applies To: Agilent 5200, 5300 and 5400 Fragment Analyzer systems
Issue
Separation times for the Fragment Analyzer for each array format and kit.
Resolution
Caution : The times in this article are the length of time...
This Information Applies To: Agilent 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer, Oligo Pro II, ProteoAnalyzer systems
Issue
Guidance for installing the Agilent parallel capillary electrophoresis instrument controller and/or...
This Information Applies To: OpenLab CDS (version 2.x) system
Issue:
This article explains how to export OpenLab CDS 2.x data as Archived file.
For copying OpenLab CDS 2.x data to another Projects or another OpenLab CDS systems, you can use data...