This Information Applies To: Agilent 5200, 5300 and 5400 Fragment Analyzer systems
Issue
Separation times for the Fragment Analyzer for each array format and kit.
Resolution
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Caution: The times in this article are the length of time for the separation section within each default method. The times are not how long it will take to run the entire default method. The entire default methods will take longer due to, for example, the conditioning and prerun steps included in the method.
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Separation times for the Fragment Analyzer quantitative kits are detailed in Table 1.
| NGS and Small Fragment | Large Fragment | Genomic DNA | RNA | ||||||||||
| DNF-473 | DNF-474 | DNF-476 | DNF-477 | DNF-464 | DNF-492 | DNF-467 | DNF-468 | DNF-488 | DNF-470 | DNF-471 | DNF- 471 extended | DNF- 472 | |
| 12 Capillary Ultra Short Array | 25 | 25 | 33 | 33 | 35 | 30 | 50 | 50 | 30 | 18 | 31 | NA | 31 |
| 12 Capillary Short Array | 50 | 50 | 45 | 40 | 55 | 50 | 60 | 60 | 50 | 24 | 40 | 90 | 40 |
| 12 Capillary Long Array | 80 | 80 | 75 | 75 | 95 | 80 | NA | NA | 80 | 45 | 70 | NA | 70 |
| 48 Capillary Short Array | 50 | 50 | 45 | 40 | 55 | 50 | 60 | 60 | 50 | 24 | 40 | 90 | 40 |
| 96 Capillary Short Array | 50 | 50 | 45 | 40 | 55 | 50 | 60 | 60 | 50 | 24 | 40 | 90 | 40 |
| 96 Capillary Long Array | 80 | 80 | 75 | 75 | 95 | 80 | NA | NA | 80 | 45 | 70 | NA | 70 |
Table 1. Fragment Analyzer quantitative kit separation times (minutes)
Separation times for the Fragment Analyzer quantitative kits are detailed in Table 2.
| DNF-905 | DNF-910 | DNF-915 | DNF-920 | DNF-930 | DNF-935 | DNF-940 | DNF-910 CRISPR Discovery | |
| 12 Capillary Ultra Short Array | 28 | 22 | 24 | 27 | 18 | 15 | NA | 35 |
| 12 Capillary Short Array | 60 | 45 | 50 | 50 | 30 | 20 | 30 | 50 |
| 12 Capillary Long Array | 80 | 70 | 80 | 80 | 75 | 35 | NA | NA |
| 48 Capillary Short Array | 60 | 45 | 50 | 50 | 30 | 20 | 30 | 50 |
| 96 Capillary Short Array | 60 | 45 | 50 | 50 | 30 | 20 | 30 | 50 |
| 96 Capillary Long Array | 80 | 70 | 80 | 80 | 75 | 35 | NA | NA |
Table 2. Fragment Analyzer qualitative kit separation times (minutes)
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Learn how to effectively maintain and operate your Fragment Analyzer:
5200, 5300, and 5400 Fragment Analyzer System Manual on Agilent.com ProSize Data Analysis Software User Manual on Agilent.com |
For Research Use Only. Not for use in diagnostic procedures.
PR7001-2747
