This Information Applies To: Agilent 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer, and Oligo Pro II systems
Issue
The volume of sample used by Agilent parallel capillary electrophoresis systems during a run.
Resolution
The Agilent validated methods use electrokinetic (voltage) injection, for which nucleic acid enters the capillaries without reducing the sample volume. An electrical current is then used to move the nucleic acids through the gel matrix. After the run, the samples have a slightly lower concentration due to the loss of nucleic acids injected into the capillaries. It may still be possible to use the postrun samples for downstream applications.
For the Fragment Analyzer, Femto Pulse, and ZAG DNA Analyzer, it is possible to select vacuum injection (hydrodynamic injection) of the sample when creating a custom method. With vacuum injection, some sample volume would be injected from the sample plate into the capillaries. However, it is not possible for Agilent to say how much sample is taken up as Agilent has only tested the validated methods that use electrokinetic injection.
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Caution: Agilent cannot guarantee the accuracy of data generated with custom methods as Agilent has not validated them.
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Learn how to effectively maintain and operate your Agilent parallel capillary electrophoresis system:
5200, 5300, and 5400 Fragment Analyzer System Manual on Agilent.com
Oligo Pro II User Manual on Agilent.com
Prosize Data Analysis Software User Manual on Agilent.com
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For Research Use Only. Not for use in diagnostic procedures.
PR7001-2834
