For Cell Mito stress test, Seahorse Cell Mito Stress Test Kit, the recommended working concentrations are 1.5 µM for Oligomycin and 0.5 µM for Rot/AA. The FCCP concentration needs to be optimized (0.5 µM-2 µM). The optimal final compound concentration…
In general, the %CV across a group and from plate to plate should both be < 20%. Higher CVs in this case was due to edge effect on outside of the plate.
GC/MS Photo Parts List for SQ and TQ sources
Stainless Steel, Inert, Extractor, HydroInert, Chemical Ionization, and the High Efficiency Source
These diagrams and parts lists are included in the Troubleshooting & Maintenance manual for the 5977 which…
The free Glycan Peak Assignment Tool was designed to provide additional confidence in identifying InstantPC or 2AB N-glycan peaks by predicted retention times of over 100 glycans. The new tool does not require users to upload sensitive data from N-glycan…
This Information Applies To: 4150 TapeStation, 4200 TapeStation
Issue
How to deal with a TapeStation Electrode Cartridge change is due warning message.
Background
The Agilent TapeStation controller software will notify users to replace the...
This Information Applies To: Agilent OpenLab CDS
Issue
Sometimes the integration result is not ideal for all injections. This article outlines the step-by-step instruction to manually integrate peaks.
Background
There are 2 ways to integrate...
This Information Applies To: Agilent OpenLab CDS , Agilent OpenLab ChemStation
Issue
Calculation of Area percent or Height percent for uncalibrated compounds in OpenLab software. If all components respond equally in the detector, then Area% and...
This Information Applies To: Agilent ICP-MS (7700/7800/7850/7900/8800/8900)
Issue
How to power off the Agilent ICP-MS Foreline Pump to perform maintenance, or when fully powering off the Agilent ICP-MS System.
Background
The ICP-MS MassHunter...
This Information Applies To: Agilent ICP-MS (7500,7700,8800)
Issue
How to check the E2M18 or E2M18F foreline pump to determine if the pump needs maintenance?
Background
The oil cleanliness and oil level in the E2M18 or E2M18F foreline pump...
This Information Applies To: Agilent ICP-MS (7700/7800/8800)
Issue
How to replace the foreline pump oil in the DS 402.
Background
The DS 402 oil should be replaced at least every 3 months or when the oil color indicates it needs to be changed...
This Information Applies To: Agilent 8890/8860 GC Systems
Issue
How to delete data that is stored on the GC instrument.
Steps to follow
Caution : Before deleting data from your GC, Agilent recommends extraction of data as a backup...
This Information Applies To: 5200, 5300 and 5400 Fragment Analyzer, Femto Pulse and ZAG DNA analyzer
Issue
When no data is available in the Agilent ProSize data analysis software for one or more capillaries, the capillary(ies) could be misaligned...
This Information Applies To: Agilent ICP-MS (7800/7850/7900/8900)
Issue
How to check the MS40+ or MS40-S foreline pump to determine if the pump needs maintenance?
Background
The oil's cleanliness and level, in the MS40+ or MS40-S foreline...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
Powering on Agilent ICP-MS Systems after maintenance, long-term shutdown, or for power cycling.
Steps to Follow
Confirm that the ICP-MS power plug...