How do I convert the Peak Area (pA*s) measurements in a GC-FID lab report into mass/weight percent of components for analysed crude oil samples?
How do I characterise the n-paraffins and estimate the single carbon number (SCN) distribution from the…
Hi all :) For he experts here! I did my yearly maintenance (new gold seal, liner, column end cut to inlet and FID, new gas filters, ...). But since then, everytime there's a temperature change in the GC, the signal will show a sudden bump and then it…
Hello MikeL ,
Just wanted to post what we discussed during your call with the support center so if anyone else sees a similar problem they will have some things to try.
There can be issues with copying and pasting fairly large amounts of information…
I'm running a GC(7890A) / MS(5975C) with MassHunter Acquisition software. I automate much of my data analysis in Python by exporting the GC signal (FID) to .csv, and then exporting the MS signal using 'Export 3-D Data...' in MSD Productivity Chemstation…
i had faced this troubleshooting before
it is probably a software problem.
first close masshunter and double click ( remove masshunter process)
open the soft again and try to create another method with same parameters it should work
also try to…
Recently, we connected the column (CP7429) to TCD and FID using a Y splitter. However, there is no signal response on both TCD and FID after manually injection of the calibration gas (containing H2, CO, CH4, C2H4, C2H6 in CO2). The carrier gas is Ar.…
When performing the Eukariote Total RNA test on the same chip we had some samples with very degraded RNA and one sample with what seems like good quality RNA but the software failed to give a RIN value for that sample. Is there a way to still measure…
Dear Colleagues, apologizing for frequently wondering but I first faced this problem. GC produces a signal without turning on the FID Detector!? Please look at the attached photo. After turning on the detector and then conditioning the column while the…
For many FID methods, 10-20 Hz data rate is sufficient unless you're doing a fast method of some sort. You need enough data points to define the peak correctly and not get any spectral tilt. Typically 15-20 points across the peak is enough.
Hello AGILENT family,
i have a few questions on the analysis of Pb in blood samples.
am failing to get the right concentrations of the QC sample 8ppb instead it’s reading 0.08 ppb and at the same time it’s also showing overange (0.08o) even when my…
This Information Applies To: Agilent 7890A and 6890 Gas Chromatography Systems
Issue
Agilent Nitrogen Phosphorus Detectors (NPDs) use three different types of beads with different characteristics. The NPD method settings within the GC method are...
This Information Applies To: Agilent Binary and Quaternary LC Pumps
Issue
When performing a gradient analysis, it’s important to know what component determines composition if it becomes necessary to troubleshoot chromatographic inconsistencies....
This Information Applies To: Agilent ProSize, Fragment Analyzer, Femto Pulse, and ZAG Analyzer
Issue
The ladder, marker, and sample peaks in the affected run have taken considerably longer to migrate than expected.
Late migration is most often...
This Information Applies To: Agilent 4150 and 4200 TapeStation systems.
Issue
The TapeStation controller software displays a warning message, notify users to replace the needle after 3840 piercings. If the maximum number of 4480 pierces is reached...
This Information Applies To: Agilent AriaMx Reall-time PCR system
Issue
After importing data from a LIMS file or opening a template that has been created for a different dye configuration, the following message could be displayed: " Dye Configuration...
This Information Applies To: Agilent AriaMx
Issue
IQ/OQ services for the AriaMx system are not available.
Resolution
If interested, please inquire with your Sales/Services contact on the Functional Check Service, optional procedure of the...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx real-time PCR optical modules
Issue
The AriaMx has the capacity to accommodate six optical modules, and the scanning optics configuration ensures distinction between fluorescent...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Can I use reaction volumes larger than 30 μL on the AriaMx?
Resolution
There is no problem with using higher reaction volumes, but the instrument performance can only be...
This Information Applies To: Agilent OpenLab CDS software version 2.3 or higher.
Issue
Chromatogram gridlines can help users to get a better understanding of the scale of the peaks. However, in some cases, the gridlines are not desired, such as...
How to Reset the Agilent GC/MSD Tune File to Default Values
Multiple Results
This Information Applies To: Agilent GC/MS MassHunter Acquisition Software
Issue
If there is a problem with the current tuning parameters and a need to tune from the default parameters, see the following procedure to reset the EI tune file (atune...