This Information Applies To: Agilent AriaMx real-time PCR system
Can I use reaction volumes larger than 30 μL on the AriaMx?
There is no problem with using higher reaction volumes, but the instrument performance can only be guaranteed when volumes within the recommended 10-30 μl range are used.
The recommended maximum volume of 30 μl is the result of the fact that the AriaMx was designed for fast-cycling. With larger reaction volumes, you would need to add longer plateau times because higher volumes of liquid will take longer to equilibrate to temperature. For reaction volumes up to 65 μl it should work as log as you add extra time for the plateau steps.
For volumes above 65 μl it can become inconvenient, because the liquid level within the plate will remain partially outside the low profile heat block and then the heat transfer would not be efficient.
In any case, some optimization to the thermal profiles will be required.
For Research Use Only. Not for use in diagnostic procedures.