Hello, I am troubleshooting a leak in the primary pump head of an Agilent 1290 G4220A binary pump. I am visibly observing that when the pump is operating, mobile phase is leaking into the seal wash lines. Also, by looking at the detector signals, I can…
Thanks for this.
To make sure I'm understanding this correctly does this mean that the instant pilot is acting as the terminating end of the bus and that the socket that the instant pilot is connected to is the one that is failing when no instant pilot…
I have observed that when a method is transferred to a new system, you need to save it in the new system before using it. If you just copy the method and use it, sometimes it fails. Just by saving it, it makes it work. I guess it saves some defaults from…
We have an LC-MS with an Agilent 1260 Infinity HPLC stack, with a G7167A multisampler. The multisampler has one drawer installed with 2x 54-vial plates. We have had some communication issues recently where the transport initialistation kept failing after…
Hello, To cut to the chase: No, peak areas calculated in MassHunter will not be equal to the peak areas calculated in GC/OpenLAB ChemStation software. GC ChemStation and OpenLAB ChemStation use response units of pA for FID data whereas the response axis…
What are the detector voltages? Select the Hardware button, or Hardware/Dashboard from Task manager, and click the Detector image. Select EM setting. What are the voltages for Discriminator, Analog HV, and Pulse HV?
Was the instrument off, for an extended…
Hi, My method for 6890 GC/FID has a warning message that there are overlapping integrations for some of the compounds, I would like to correct this but unsure how, I know the answer lies in the integration events table. Any help is appreciated!
it does not work,
I tried to install openlab v2.4 manually, the installation failed at the agilent platform in the install or upgrade content management part,
Hi Lycrecia,
We checked the volume calibration section and we did the full range volume dispense, as described in the manual. However, we are not sure which value we should add to the actual volume in the volume calibration screen. Do we need to do…
It is great you have such a nice experience with your results, however results accuracy is a matter of hardware robustness and method setting, not software. I your insist on this, I can recommend you to do the following steps:
1- get the licence number…
This Information Applies To: Agilent 2100 Bioanalyzer system
Issue
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Background
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real...
This Information Applies To: Agilent 4200, 4150 and 2200 TapeStation.
Issue
Is it possible to analyze single stranded DNA (ssDNA) on the TapeStation? Which kit is the most suitable for analysis of ssDNA?
Resolution
Agilent does not offer any...
This Information Applies To: Agilent 4200 and 4150 TapeStation systems
Issue
How to Run the software installation verification on 4200 and 4150 TapeStation systems.
Background
The 4150 TapeStation model G2992A and 4200 TapeStation model G2991B...
This Information Applies To: Agilent 5200/5300/5400 Fragment Analyzer, and Femto Pulse
Issue
Perform a capillary alignment when a new array is installed in your Agilent Parallel Capillary Electrophoresis instrument. An alignment may also be performed...
This Information Applies To: Agilent 4200 and 4150 TapeStation
Issue
Are the Agilent DNA and RNA ScreenTape assays denaturing?
Resolution
The DNA ScreenTape assays in the TapeStation portfolio are not denaturing. Only the RNA and High Sensitivity...
This Information Applies To: Agilent LC pumps with manual purge valve.
Issue
A good practice before using a system is to purge the system to remove air. This is done by observing the clear tubing from the solvent bottle as well as the waste line...
This Information Applies To: ICP-MS MassHunter 4.x and 5.x
Issue
Unable to open a batch in offline data analysis, or the batch is empty.
If the ICP-MS MassHunter software closes unexpectedly, there is a power outage, or the ICP-MS and computer...
This Information Applies To: Agilent OpenLab CDS versions 2.4 or higher.
Issue
How to preview result files using various Report Templates in OpenLab CDS versions 2.4 or higher.
Requirements
The user has the result set loaded in OpenLab CDS...
This Information Applies To: ICP-MS MassHunter with Spectroscopy Database Administrator (SDA) and Protect Local Data ON.
Issue
An 'Authentication failed' error message appeared after entering user name and password when attempting to download a...
This Information Applies To: ICP-MS MassHunter with Spectroscopy Database Administrator (SDA) and Protect Local Data On.
Issue
With Spectroscopy Database Administrator (SDA) and Protect Local Data turned on, batches are not accessible through file...