I had notice that the air volume is set to 1ul which is the same as the sample volume (I’m assuming is the same as the injected volume). You could try to set the air volume to 0ul and observe if the injection is consistent after this.
Hi Susana,
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Hi Mike, Thank you, yes correct, here is our situation to clarify, we upgraded in order to retire the Win7 OS. We had methods on Agilent UV 8454 ChemStation B.05.02 that managers would create, reviewers would approve, and analyst could use the methods…
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Wow, your peaks are super close to each other! Anything minor will affect your chromatogram. Maybe you should search for a better method? Anyways, what impacts the most are flow (are you running a gradient method?), organic concentration (again, gradient…
You can calculate the pressure on the flow cell by disconnecting the inlet capillary in the VWD. So the pressure in the VWD is the system pressure MINUS the pressure without VWD
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This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx touch screen displays error code 90 after the Instrument Health Check (IHC), which happens when the AriaMx is switched on, or after running the onboard diagnostic tests...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx optical modules
Issue
AriaMx users can change which AriaMx optical modules are installed to upgrade the instrument capabilities or to troubleshoot.
Items Required
Any...
This Information Applies To: AriaMx real-time PCR system
Issue
Error 43 is displayed on the AriaMx touch screen. Error 43 is a voltage error and it normally indicates that the cartridge LED voltage or motor voltage being detected is abnormal. Sometimes...
This Information Applies To: Agilent 2100 Bioanalyzer system
Issue
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Background
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real...
This Information Applies To: Agilent 4200, 4150 and 2200 TapeStation.
Issue
Is it possible to analyze single stranded DNA (ssDNA) on the TapeStation? Which kit is the most suitable for analysis of ssDNA?
Resolution
Agilent does not offer any...
This Information Applies To: Agilent 4200 and 4150 TapeStation systems
Issue
How to Run the software installation verification on 4200 and 4150 TapeStation systems.
Background
The 4150 TapeStation model G2992A and 4200 TapeStation model G2991B...
This Information Applies To: Agilent 5200/5300/5400 Fragment Analyzer, and Femto Pulse
Issue
Perform a capillary alignment when a new array is installed in your Agilent Parallel Capillary Electrophoresis instrument. An alignment may also be performed...
This Information Applies To: Agilent 4200 and 4150 TapeStation
Issue
Are the Agilent DNA and RNA ScreenTape assays denaturing?
Resolution
The DNA ScreenTape assays in the TapeStation portfolio are not denaturing. Only the RNA and High Sensitivity...
This Information Applies To: Agilent LC pumps with manual purge valve.
Issue
A good practice before using a system is to purge the system to remove air. This is done by observing the clear tubing from the solvent bottle as well as the waste line...
This Information Applies To: ICP-MS MassHunter 4.x and 5.x
Issue
Unable to open a batch in offline data analysis, or the batch is empty.
If the ICP-MS MassHunter software closes unexpectedly, there is a power outage, or the ICP-MS and computer...