Andy G. had the excellent suggestion to decrease draw speed and wait time, since we are working with methanol extract. So we halved the draw speed from 100ul/min to 50ul/min and increased the wait time from 2 to 3 seconds. For now, this seems to have…
Hi guys, when I tried to run my sequence in GC-FID, the workstation showed "front injectors not detected", and under my method, the configuration showed no injectors. However, the parameters I entered for injection could be found in the GC (7890A) operation…
Hi everyone
In my company recently we update the Openlab Ezchrom to CDS 2,5.
We have a GC 7820A and when we analyzed Residual dissolvents we have a trouble because between 3 to 5 minutes the detector (FID) get shutdown and this signal of shutdown…
Hi Abdul,
From the picture, it appears your FID flame was extinguished. You can put a metal wrench or a mirror at the top of the detector to see if the exhaust contains moisture from the combustion, but a flat baseline at zero output usually means the…
This instrument is used mostly for FID analyses and the second injector is not working. That's the primary reason why the column will be disconnected most of the time. Also to reduce contamination of the ECD, but it sounds like that isn't a huge concern…
Good Day.
I have an issue to my result in tcd. My Sample is TOGA using Fid and tcd. now the issue is the detection of H2, N2 and O2 are late. before 4.2 min. now its 6.2. the column is new.
Thank you for the reply, I will have a go at it tomorrow. So essentially I just need to change one of my internal standard compounds to a normal compound, and use that as a single point calibration? It just seemed logical that every internal standard…
We need to use air generator for 8 GCs with FID Detector. Each FID uses 350 mL/min air. So for 8 FID, 2.8L air will be sufficient? Is it ok to go for an air generator that supply 3L of air/min or we need to go for a higher option? Kindly advise
In an official Service Note we say "According to our lamp manufacturers there is no shelf life for LC detector deuterium (D2) lamps. Our logistic centers (LC) try to ship lamps from stock before they reach 12 months age. We see the lamps lasting for more…
What version of Windows do is it running? Go to the cell next to the logo (Type here to search) and enter winver and select the run app. At top it should say Windows 10, at this time only MassHunter 5.2 is Win11 compatible. 2 lines below Windows 10 will…
This Information Applies To: Agilent 6820, 6890, 7820A GC systems with a Split/Splitless (SSL) inlet
Issue
Non-volatile sample matrix components will accumulate in the inlet liner and impact on analytical performance. The inlet liner should be changed...
This Information Applies To: Agilent headspace samplers (HS)
Issue
GC cycle time needs to be calculated correctly. Too short a GC cycle time will reduce sample quality and possibly stop the analysis prematurely. Too long GC cycle time can cause...
This Information Applies To: Agilent 7820A GC
Issue
7820A GC is designed with simplified keypad that comes with five buttons. They are Stop, Prep Run , Start, Up and Down arrow buttons. Changing the IP address requires a different method compared...
This Information Applies To: Agilent Single Needle Autosamplers
Background
Sometimes the user may observe liquid flowing from Port four on the autosampler injection valve. During an injection sequence, it is normal to have liquid from port four...
This Information Applies To: Agilent 4200, 4150, 2200 TapeStation systems
Issue
Can I run an assay using the ladder or sample buffer from a different assay if I have run out of the appropriate assay ladder or sample buffer? Is it possible to run...
This Information Applies To: Agilent ICP-MS 7700, 7800, 7850, 7900, 8800, and 8900 systems
Issue
Peristaltic pump tubing that has lost its elasticity will not deliver the sample to the nebulizer correctly. When the pump tubing appears limp or flattened...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx touch screen displays error code 90 after the Instrument Health Check (IHC), which happens when the AriaMx is switched on, or after running the onboard diagnostic tests...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx optical modules
Issue
AriaMx users can change which AriaMx optical modules are installed to upgrade the instrument capabilities or to troubleshoot.
Items Required
Any...
This Information Applies To: AriaMx real-time PCR system
Issue
Error 43 is displayed on the AriaMx touch screen. Error 43 is a voltage error and it normally indicates that the cartridge LED voltage or motor voltage being detected is abnormal. Sometimes...
This Information Applies To: Agilent 2100 Bioanalyzer system
Issue
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Background
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real...