The file is a pre-set qPCR program to run a plate filled with water used to test the basic raw fluorescence signal of AriaMx Optical modules.
File required for Performing a Background Calibration
The GC cycle time should be the time from injection until the GC is ready to run again. This includes the analysis run, cool down, and equilibration. Based on the times you listed, it would be roughly 40 minutes. It may be worth timing to make sure. The…
Hi howard_sanford, my apologies for a slow response, but thank you very much for the help and advice. I was able to get the filter for Cal and CC to work, and I appreciate the discussion of my larger problem. I think I'm probably several (many) steps…
Hello, thank you for your help. I had tried loading different processing methods but I didn't realize the results were not linked to any method. It turns out the result files were read-only and could not be linked to any method. I moved the result files…
The peaks look similar, essentially the same sensitivity. I'd look at the background on your older runs and the background now. It could be that most of the previous background was one, two, a few, ions and they're gone now. Was that small but still noticeable…
That is one failure mode of Platinum Resistance Thermometers. It can happen one degree to the next higher degree. The heater/sensor must be replaced, part number G1099-60107.
See also: (+) AUX 2 heater fault - Forum - GC/MS - Agilent Community
This Information Applies To: Agilent OpenLab ChemStation
Issue
When launching OpenLab ChemStation Online or Offline session, the following error messages “ ChemStation ChemMain has stopped working ” or " There is a problem with Adobe Acrobat/Reader...
This Information Applies To: Agilent 5200/5300/5400 Fragment Analyzer, Agilent Femto Pulse, Agilent ZAG DNA Analyzer
Issue: The instrument encounters a run error, Run Aborted, Position fault: Did not reach target Z position ( Figure 1 ) . This error...
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG, Oligo Pro II
Issue:
This article explains the steps to take after installing a brand new array in the Agilent 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG, and...
This Information Applies To: Agilent 5200/5300/5400 Fragment Analyzer systems, RNA kit (15 nt) (p/n DNF-471-0500/1000), HS RNA kit (15 nt) (p/n DNF-472-0500/1000), Small RNA kit (p/n DNF-470-0275)
Issue
Which kit should be used to analyze single...
This Information Applies To: Agilent 6820, 6890, 7820A GC systems with a Split/Splitless (SSL) inlet
Issue
Non-volatile sample matrix components will accumulate in the inlet liner and impact on analytical performance. The inlet liner should be changed...
This Information Applies To: Agilent headspace samplers (HS)
Issue
GC cycle time needs to be calculated correctly. Too short a GC cycle time will reduce sample quality and possibly stop the analysis prematurely. Too long GC cycle time can cause...
This Information Applies To: Agilent 7820A GC
Issue
7820A GC is designed with simplified keypad that comes with five buttons. They are Stop, Prep Run , Start, Up and Down arrow buttons. Changing the IP address requires a different method compared...
This Information Applies To: Agilent Single Needle Autosamplers
Background
Sometimes the user may observe liquid flowing from Port four on the autosampler injection valve. During an injection sequence, it is normal to have liquid from port four...
This Information Applies To: Agilent 4200, 4150, 2200 TapeStation systems
Issue
Can I run an assay using the ladder or sample buffer from a different assay if I have run out of the appropriate assay ladder or sample buffer? Is it possible to run...
This Information Applies To: Agilent ICP-MS 7700, 7800, 7850, 7900, 8800, and 8900 systems
Issue
Peristaltic pump tubing that has lost its elasticity will not deliver the sample to the nebulizer correctly. When the pump tubing appears limp or flattened...