Use 1 meter of deactivated fused silica tubing going into the source (the same diameter of your column), then use a union (either the press-fit glass type of, better yet, the SilTite brand) to connect to your column in the oven. When you want to change…
Hello tdnola , "in use by another..." is a safety function that prevents a second session to the MSD: OK & wanted function. 1st) troubleshooting because a PING does only a small (OSI) communication check (are voltages & cabling OK?), I recommend the communication…
Hi community,
maybe someone has an idea what I could do, before a technician comes over.
During my absence my colleagues told me that our HPLC (1260 Vialsampler, 1260 Inf II Flex pump, TCC, FC-AS, DAD) does show a peak shift of about 3 to 10 minutes…
Hello everyone,
I have created a simultaneous SIM/scan method and we're using a HS-SPME-GC-MS system (GC: Agilent 7890 A GC-System; MS: Agilent 5975C Inert XL MSD with Triple-Axis Detector; Software: Chemstation G1701EA E.02.00.493).
We get a TIC…
Were you able to join us on April 11th for our Agilent GC Live session? If so, we would like to hear your thoughts and questions. If you missed it, please see the video below.
Time stamps refer to the above video.
(14:05) What is…
Yes, it is the constant flow mode. The following is the method I am using: Carrier gas: Helium Carrier gas flow rate: 1 mL/min (constant flow) Injection volume: 1 μL Injection port temperature: 250 °C Split injection ratio: 30:1 Initial oven temperature…
We work with a Q-TOF 6545 and it fails positive auto tune. Is there anyone who knows this error, what causes it and what can be done to rectify this? Thank you
Hello, I am trying to generate a streamlined intelligent report in OpenLab where we can capture all peaks of our SEC gel filtration standard in a single table and be able to evaluate APD Pass/Fail for each peak and Resolution between BGG and Ovalbumin…
Thanks for the feedback. I have re-optimized the lamp alignment, but I didn’t get much (if any) improvement. I also made some adjustments to the sample position, which I think helped a little. I think some users have been fiddling with the sample position…
This Information Applies To: 4200, 4150 and 2200 TapeStation, TapeStation analysis software A02.02 or higher.
Issue:
When using the Agilent 4200, 4150 and 2200 TapeStation systems, the DV 200 of FFPE RNA samples can be calculated within the TapeStation...
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer, ProSize Data Analysis Software
Issue
How to export a ladder as a size calibration file and import it into a different run in ProSize.
Background
...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
It is possible to set up the 2100 Expert software so that the files are automatically exported each time a chip run is complete.
Steps to Follow
To turn...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software
Issue:
How to identify and troubleshoot late migration of the ladder, marker, and sample peaks in data generated on the Agilent 2100 Bioanalyzer system.
Background
...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
If communication between the 2100 Bioanalyzer and the PC cannot be established, or if an intermittent communication loss occurs, follow the instructions in...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
Instructions on how to import from or export data into LIMS software.
Steps to Follow
Data import
When importing sample names and sample comments from...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software version B.02.08 or later
Issue
When printing a report to PDF or HTML with the 2100 Expert software, the error "Failed to Generate Report" appears.
Background
This error...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software
Issue
Resetting upper or lower markers from their automatically assigned settings in the Agilent 2100 Expert software.
Background
The Agilent 2100 Expert software...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert Software
Issue
Samples with a broad signal such as NGS smears should be analyzed by setting up a region. Analyzing a region is also termed smear analysis.
Steps to Follow...