Raman water sensitivity fail

Dear Agilent Community.

 

We have a Cary Eclipse fluorescence spectrophotometer (part number 02-101669-00, serial number EL 0604-3601) that is failing the Raman water sensitivity tests at both 350 and 500 nm excitation.

 

It passed all the tests when a new lamp was installed in 2016.

 

Is this a indication that it may be time for a new lamp?

 

Is there a counter that logs the lamp hours?

 

Is there a test specifically to determine the performance of the lamp?

 

We're using WinFLR 1.2 (147) under Windows 10 32-bit.

 

Ciao,

Chappy.

Parents Reply
  • Thanks for the feedback.

     

    I have re-optimized the lamp alignment, but I didn’t get much (if any) improvement.

     

    I also made some adjustments to the sample position, which I think helped a little.  I think some users have been fiddling with the sample position.

     

    The Raman sensitivity test is now passing with 500 nm excitation (597:1, reference 500:1), but still failing with 350 nm excitation (528:1, reference 750:1).

     

    The lamp is almost 1000 hours old.

     

    Is there a recommended lifetime for the Cary Eclipse lamps?  They are not just the lamp.  They are the entire housing.

     

    Ciao,

    Chappy.

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