I see the clear difference between the split mode vs the splitless that now has the valid parameter. and the initial nitrogen elution seems a lot less problematic. thank you for your instructions!
Hi rguzmans1
To prepare a litre of this mobile phase, weigh 0.315g of ammonium formate and dissolve in 800mL of water, add 100µL formic acid and mix well, then make up to total volume of 1L.
Probably best to filter this after preparation through 0…
Hello ivynotterpek ,
I'm not aware of any way to do this with just one compound/cal curve. One solution would be to make duplicate compounds and assign one compound the low level standards and the other the high level standards. You can utilize different…
Thank you, unfortunately we tried to set a collection window much larger than the peak duration, but the peak got split again. And in the version of the software we are using no advanced settings allowing to collect different peaks into the same vial…
Hi CMZainea,
Thank you for your feedback. The leak test for the 6 ports valve was passing, so no leaks detected at the valve. Any other leaks were detected from the visible piping and connection from the back panel of the 7697A going to the 6 ways valve…
This Information Applies To: Agilent OpenLab CDS 2.x
Issue
This article outlines the step-by-step instruction on how to overlay chromatograms for OpenLab CDS 2.x
Steps to Follow
Load data from result sets or single samples. See Load or add...
This Information Applies To: Agilent Lab Advisor Software, Agilent Multicolumn Thermostat
Issue:
Agilent Lab Advisor is a great tool to diagnose the issue of your HPLC. Running the tests that are relevant to your specific issue in the Service &...
This Information Applies To: Agilent OpenLab CDS (ver 2.x)
Content:
The following procedure shows how to review completed injections in a running sequence in OpenLab CDS (ver 2.x).
Steps to Follow:
Select the sequence from the Run Queue...
This Information Applies To: Agilent Liquid Chromatography (LC) systems
Issue:
Shutting down the HPLC for a long period requires following this general procedure to prevent damage to the system and the column.
Steps to follow:
Store the...
This Information Applies To: Agilent G7129A/B Autosampler with Sample Thermostat/Cooler G7167-60101 or G7167-60005.
Issue:
The autosampler may stop working from time to time, potentially giving off loud noises and switching off the light. The only...
This Information Applies To: Agilent 5977A/B GC/MSD with EI source, EI Extractor Source, or CI Source.
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte...
This Information Applies To: Agilent HES source, Agilent 5977 Series GC/MSD
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte response not improving...
This Information Applies To: Agilent 5977 and 5975 Series GC/MSD systems
Issue
The autotune fails and reports the error Tune action stopped: Gain and offset = 0 and peaks are too narrow!
Resolution
The MSD autotune program is run after routine...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
Condition new or cleaned ICP-MS skimmer cones and sampler cones by aspirating a Conditioning Solution before use.
Background
Drift up can be a sign of...