• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Analytical LC Systems Preparative LC Systems
  • Component :  LC Column Compartments LC Degassers LC Detectors LC Fraction Collectors LC Injectors and Sample Thermostats LC Pumps LC Valves
  • Product Name :  1100 Series LC System 1120 Compact LC 1200 Series LC System 1220 infinity II LC System 1260 Infinity II Bio-Inert LC System 1260 Infinity II LC System 1260 Infinity II Prime Bio LC System 1260 Infinity II SFC System 1260 Infinity II SFC/UHPLC Hybrid System 1260 Infinity LC System 1260 Infinity II Prime LC System 1290 Infinity II Bio LC System 1290 Infinity II LC System 1290 Infinity LC System
  • Task :  Best Practices Operation
Related Articles

  • No Pressure, No Flow, RT shift Issues: Cleaning Acetonitrile (ACN) Aggregations in Agilent Pumps

    This Information Applies To: Agilent LC pumps Issue: Stuck or obstructed check valves (Active Inlet Valve (AIV), Passive Inlet Valve (PIV), Outlet Ball Valve (OBV)) can cause issues such as no-flow or no-pressure, moderate to strong drops in pressure or retention time shifts. You can see the article How to Troubleshoot no flow or no pressure issues: Binary/Quaternary/Isocratic Pumps - G131XX, and G711XX to troubleshoot the issue. If one of the valves is the source of the blockage, and acetonitrile...

  • How to Clean an FID

    This Information Applies To: Agilent 6850 A/B, 6890 A/N/Plus, 7820A, 7890A/B, 8860, 8890 Gas Chromatographs with Flame Ionization Detector (FID) Issue: The cleaning of the Flame Ionization Detector (FID), may be required in the following scenarios: Preventive or periodic maintenance. Resolving performance issues of the detector: High background, noise, or spiking of baseline. Problems to ignite the flame. Steps to follow: Tools Required: Torx T20 screwdriver (p...

  • Finding Declarations of Conformity, Certificates of Conformance and Safety Data Sheets for the Agilent TapeStation Assays

    This Information Applies To: TapeStation ScreenTapes and reagents Issue Using the search tool on Agilent.com to locate the declarations of conformity (DOC) for Agilent ScreenTapes and Test-Tapes, certificates of conformity (COC) for TapeStation reagents and safety data sheets (SDS). Steps to Follow To obtain DOC or COC documents, Search for the Test-Tape ID, ScreenTape, or reagent lot number in the search field on Agilent.com . Then select the content type Certificate of Conformance...

  • Finding Declarations of Conformity, Certificates of Conformance and Safety Data Sheets for Agilent Bioanalyzer Reagents

    This Information Applies To: 2100 Bioanalyzer reagents and chips Issue Agilent publishes the declarations of conformity (DOC) for Agilent Bioanalyzer reagents and certificates of conformance (COC) for Bioanalyzer microfluidics chips on Agilent.com . The DOC and COC have been published on Agilent.com for every lot produced since May 2021. To improve the reagents environmental footprint, Agilent removed the hard-copy of the DOC, COC, and the document providing the link to the safety data sheets...

  • Reporting Peak Area and Retention Time of Sequence in Excel Format with OpenLab CDS

    This Information Applies To: Agilent OpenLab CDS Issue This article shows how to export a report of peak area and retention time of a sequence in an Excel format. Prerequisites To be able to carry out the procedure as described, privileges of Data Processing > Print results reports and Report Template > Create report template are required. Privileges are configured in the Control Panel . The relevant peaks in the chromatograms are integrated. Steps to Follow Identify peaks...

How to Prepare your HPLC for a Long Time Shut Down

Answer

This Information Applies To: Agilent Liquid Chromatography (LC) systems

Issue:

Shutting down the HPLC for a long period requires following this general procedure to prevent damage to the system and the column.

 

Steps to follow:

  1. Store the column in an appropriate solvent (check column information sheet)
  2. When you remove the column, add a column union so the detector gets flushed as well in the following steps.
  3. For a system in reversed phase -> Purge all lines with hot HPLC-grade water (60 to 70 °C) to remove salts or ACN polymeric by-products.
    You may use the following solvents for storing the instrument: 100% HPLC grade IPA (Isopropanol), IPA: Water mix > 10:1 or a (50:50) MeOH: Water mix. No salts should be left in the system.
  4. For a system in Normal phase, do not purge with water -> purge with IPA directly and then move on to store in 100% HPLC grade IPA or IPA: Hexane mix > 10:1.   
  5. Fill all solvent lines with IPA or the storing solvent.
  6.  Plug (or seal) all open connections.
  7. Emptying waste containers and mobile phase bottles is recommended to avoid any contamination later on. 
  8. Power off all modules and the PC / control software. 
  9. If you have an evaporative light scattering detector (ELSD), close all regulators and the gas tank. 

 

 Tip 
Learn how to maintain your Agilent LC system:
Collection of LC (HPLC) Resources in the Agilent Community

 

 
Was this helpful?
Contact Agilent Support

Still Need Assistance?

Contact Agilent Support >
Privacy Statement
Terms of Use
Contact Us
Site Help