Hello, H2 is the fuel for the flame in the FID, so if you are out of H2 then the FID will not be able to light. That is very likely the reason why you are not seeing a signal from the FID.
I have a 6490 QQQ. Each time I run either autotune or check tune, I get masses that are out of tolerance especially in MS2. The system seems to pass if the Dynamic Ramp Table enable button is checked. This is associated with the collision cell and detector…
I’m sorry for the delayed reply to your message. Do you still need assistance with this? If you do, please take into consideration the notes below. However, if the issue was resolved, could you please share what the resolution was?
From what I understand…
Based on your latest results, I would say that nothing is fundamentally wrong with the optical unit in terms of noise, so I believe this is more an applicational problem - your passed quick noise test points to that direction. The error 1050 with the…
No, the flows on the two columns downstream of the switch have to be equal. They are feed by the same pressure source, so since MSD is under vacuum and the FID is ambient, the column connected to the MSD has to have more restriction, to keep the flows…
I have decades of experience with consumer products, all on Agilent GC, GCMS, and HPLC.
If I was needing to quantitate 1,2-octanediol in cosmetics samples, I'd extract the sample with dimethylformamide and add BSTFA to make trimethylsilyl derivative…
Disconnect the sample probe from the valve and start the leak and restriction test. If it passes this step, it means that there is some restriction in the sample probe, enough to cause that part of the test to fail. Try a brand new probe. If it fails…
Seems then to indicate something in the flow path when including the mixer and damper. First, make sure that you do an elasticity calibration because you have changed important parts in the flow path. Then re-run the pump leak rate test with the mixer…
Hello everyone,
We have a problem with the acquisition methods on GC, but not on HPLC.
When we want to change an acquisition method that is in "approved" status to "generic" status in order to modify it, it is impossible to do so because the box is…
This Information Applies To: Agilent OpenLab CDS
Issue
Identifying the baseline codes and baseline code descriptions for OpenLab CDS
Background
OpenLab CDS Data Analysis includes the same integration algorithms as available in both OpenLAB...
This Information Applies To: Agilent OpenLab CDS
Issue
This article provides step-by-step instructions to filter a signal in an Intelligent Report.
Requirements
To be able to carry out the procedure as described, the privilege of Data Processing...
This Information Applies To: Agilent OpenLab CDS
Issue
This article outlines different ways to quantify the amount of compound in an unknown sample.
Background
Quantitation uses peak area or height to determine the amount of a compound in...
This Information Applies To: Agilent G1329A/B and G1313A Standard Autosampler
Issue
Replacing the needle seat of the Agilent 1100/1200/1260 Standard Autosampler may be required during regular maintenance, replacing a bent needle, or flow path blockage...
This Information Applies To: Agilent G1329A/B and G1313A Standard Autosampler
Issue
Replacing the needle of the Agilent 1100/1200/1260 Standard Autosampler may be required during regular maintenance, replacing a bent needle, or flow path blockage...
This Information Applies To: Agilent 1290 Infinity (G4226A) and Agilent 1260 Infinity (G1367E) HiP Autosamplers
Issue
Replacing the Needle Assembly of the Autosampler is required when:
The needle is visibly damaged.
Leaks or blockages in the...
This Information Applies To: Agilent 6000 Series LC/MS Systems equipped with MS40+ rotary vane pumps
Issue
Replacing the MS-40+ (rough pump) fluid periodically is a key maintenance procedure to improve vacuum performance and minimize the possibilities...
This Information Applies To: All Agilent 6000 Series LC/MS Systems without a gate valve for ion capillary installation/removal. For instructions for removing an ion capillary on LC/MS instruments with a gate valve installed, please click here
Issue...
This Information Applies To: Agilent 6000 series LC/MS instruments
Issue:
Cleaning the ion transfer capillary as part of regular maintenance or as part of troubleshooting when a loss of sensitivity in the instrument occurs.
Steps to follow ...
This Information Applies To: Agilent 6000 Series LC/MS systems.
Issue
Efficient electrospray ionization (ESI) in your LC/MS ion source requires a properly adjusted nebulizer needle in good condition. The nebulizer needle is a consumable item in...