Hi, the peak area has no influence on the calculation of Mw when measuring with concentration detectors only (RI, UV, ELSD e.g.). When performing Viscometry & Lightscattering measurements, the peak area is needed for the calculation. As an additional…
Occasionally the tune files get corrupted and you need to go back to an old one or start again from the default atune.u file. Glad the problem is resolved!
Part 1 included many questions and some answers about the maximum abundance that should be detected at the electron multiplier. Another important consideration is how much sample should go into the column.
See Which Electron Ionization Ion Source? Part…
Thank you for taking a moment to review the Community Guidelines. You agree to all of the guidelines posted and linked here when using the Agilent Community. These guidelines are here to help you be a valuable contributor to the community and make sure…
Hi, I have an old 6890 with a jet direct card. We lost power to the instrument and since then it has not been able to connect to the PC. I tried updating the bootp software with the correct mac address and power cycled instrument with the same issue.…
Support and educational resources for the Microplate Centrifuge and Automated Centrifuge Loader .
Automation Solutions Knowledge Base
Microplate-Handling Device Guides
PDF user guides
Support Documents
G5582A Centrifuge Installation Checklist…
-Have you tried a diferent column ? An old column could have retention time issues and peak distortion.. -Have you had any pressure fluctuations during your run ? Try running a Pump leak rate test, to see if the problem is not in the pump-head. -Do a…
This Information Applies To: Agilent LC Pumps
Issue
If a user suspects that the gradient composition isn’t accurate and causes poor chromatography such as retention time shifts, late, early, no peaks or merging peaks. This article provides a quick...
This Information Applies To: Agilent 1290 Infinity (G4226A) and Agilent 1260 Infinity (G1367E) HiP Autosampler
Issue
Replacing the Needle Seat of the Autosampler is required when:
The needle seat is visibly damaged.
Leaks or blockages in the...
This Information Applies To: Agilent OpenLab CDS
Issue
This article discusses different types of bracketing modes and how to set up a bracketed calibration in Data Analysis for OpenLab CDS 2.x
Background
Occasionally, the instrument conditions...
This Information Applies To: Agilent OpenLab CDS
Issue
Identifying the baseline codes and baseline code descriptions for OpenLab CDS
Background
OpenLab CDS Data Analysis includes the same integration algorithms as available in both OpenLAB...
This Information Applies To: Agilent OpenLab CDS
Issue
This article provides step-by-step instructions to filter a signal in an Intelligent Report.
Requirements
To be able to carry out the procedure as described, the privilege of Data Processing...
This Information Applies To: Agilent OpenLab CDS
Issue
This article outlines different ways to quantify the amount of compound in an unknown sample.
Background
Quantitation uses peak area or height to determine the amount of a compound in...
This Information Applies To: Agilent G1329A/B and G1313A Standard Autosampler
Issue
Replacing the needle seat of the Agilent 1100/1200/1260 Standard Autosampler may be required during regular maintenance, replacing a bent needle, or flow path blockage...
This Information Applies To: Agilent G1329A/B and G1313A Standard Autosampler
Issue
Replacing the needle of the Agilent 1100/1200/1260 Standard Autosampler may be required during regular maintenance, replacing a bent needle, or flow path blockage...
This Information Applies To: Agilent 1290 Infinity (G4226A) and Agilent 1260 Infinity (G1367E) HiP Autosamplers
Issue
Replacing the Needle Assembly of the Autosampler is required when:
The needle is visibly damaged.
Leaks or blockages in the...
This Information Applies To: Agilent 6000 Series LC/MS Systems equipped with MS40+ rotary vane pumps
Issue
Replacing the MS-40+ (rough pump) fluid periodically is a key maintenance procedure to improve vacuum performance and minimize the possibilities...