This Information Applies To: Agilent LC Pumps
If a user suspects that the gradient composition isn’t accurate and causes poor chromatography such as retention time shifts, late, early, no peaks or merging peaks. This article provides a quick way to determine if the pump can accurately proportion the requested composition. The test is done by manually changing the composition and noting the responses.
Note: This procedure can only be done with a UV detector, not valid for non-UV systems.
Steps to Follow
If the test fails, ensure you have followed the procedure carefully then contact your local Agilent service representative.