Does area of the peak have influence on calculation of MW in GPC/SEC software?
Does area of the peak have influence on calculation of MW in GPC/SEC software?
Hi, the peak area has no influence on the calculation of Mw when measuring with concentration detectors only (RI, UV, ELSD e.g.). When performing Viscometry & Lightscattering measurements, the peak area is needed for the calculation.
As an additional side remark: Narrowly distributed high molar masses lead to high-solution viscosities, a high concentration produces a very viscous injection band, so that the diffusion process on the column can be hindered. This results in higher elution volumes measuring lower molecular weights - this phenomena is less pronounced for low molecular samples. See the example with polystyrene below.
Hi, the peak area has no influence on the calculation of Mw when measuring with concentration detectors only (RI, UV, ELSD e.g.). When performing Viscometry & Lightscattering measurements, the peak area is needed for the calculation.
As an additional side remark: Narrowly distributed high molar masses lead to high-solution viscosities, a high concentration produces a very viscous injection band, so that the diffusion process on the column can be hindered. This results in higher elution volumes measuring lower molecular weights - this phenomena is less pronounced for low molecular samples. See the example with polystyrene below.