I am trying to run a Injector Precision PV test using the conditions described in the software: caffeine standard (125 ug/mL) and HP ODS Hypersil 5 um, 125 x 4 mm part 7982618-564 . The method uses 85:15 Water:ACN mobile phase at 0.8 mL/min. The test…
Hello mkaadan ,
What I meant by a "low" failure rate (notice the quotation marks), is that it is likely not an indication of a leak, as that would result in a lot more samples failing, or potentially all of them. By no means I wanted to minimize the…
Can SimDis data processing be performed manually (i.e. without the aid of additional SimDis software produced by Agilent or a third-party) with a GC running Chemstation?
I'm looking for a low-cost solution to perform SimDis analysis of jet-fuel samples…
Hi, I have a GC/MS (8890 GC with 5977b HES MSD) that is also configured with an FID and PDHID. I would like to create an FID/PDHID-only method, where the MSD remains off the entire run and does not collect data. I haven't figured out how to do this so…
l have lc 1260 pump type G1311c and it is in residual mode due to installing a degasser board on the pump board. I used the Lab Advisor program to update the firmware, but the process was not successful and messages appeared. Connection failed instrument…
Hi everyone. I have a problem with my 8890 GC-MS as I do not have pressure in the inlet. The issue began during the IT outage, the EPC module turned off itself hence all heated zones turned off as well. When I managed to re-establish the connection I…
Hi ISH, yes all was fine with column installation and pump down was efficient. The issue seems to be with the entrance lense offset - I loaded a previous tune and managed to run an autotune however it is failing due to too high 503/502 ratio. I've seen…
Hello Paul, it looks to me that there is too much acetylene in the gas mixture coming to the burner. This is causing the carbon build-up.
I'd suggest you first remove the absorption cell from the holder and clean this by soaking in dilute nitric acid…
HI Lars,
Yes, you can run the test on a VI. Specific to your system, a failing result could indicate a leak within the inlet or at any of the in-line connections of your GSV, so the results may not be clear without a point leak detector. I would also…
This Information Applies To: Agilent OpenLab CDS (version 2.x)
Contents:
In the following procedure, you will find steps to create a multipoint ESTD calibration method with OpenLab CDS 2.X.
Steps to follow:
1. Load the Data
a. From Data...
This Information Applies To: Agilent MassHunter Quantitative Analysis Software
Issue
Chromatographic peak integration is a critical part of accurate and reproducible quantitative data analysis. The integrator type and the integration parameters...
This Information Applies To: Agilent 6200/6400/6500 Series LC/MS Systems
Issue
Instructions on how to fill the calibrant bottle on 6200/6400/6500 Series LC/MS Instruments when calibrant levels are running low.
Background
The ability to autotune...
This Information Applies To: All Agilent 6000 Series LC/MS Systems without a gate valve for ion capillary installation/removal.
Issue:
Installing the Ion Transfer Capillary after cleaning or replacement. For instructions for installing an ion capillary...
This Information Applies To: AriaMx Real-time PCR System
Issue
The saturation level is unique for each optical module after the offset of calibration factor and background calibration. The FAM, ROX, HEX, CY3 & CY5 optical modules will saturate at...
This Information Applies To: Agilent LC Systems
Issue
While it is possible to combine modules from different HPLC series such as 1100, 1200, 1260 or 1290 series to create a functioning LC system, there are some important topics to consider.
...
Fixes for the "RPC Server Unavailable" Error
Multiple Results
This Information Applies To: Agilent OpenLab CDS
Issue
When launching the instrument Acquisition screen/instrument session, there is an "RPC Server Unavailable" error.
Resolution
Accessing the Services App (Windows)
There are two ways to...
This Information Applies To: Agilent LC Pumps
Issue
If a user suspects that the gradient composition isn’t accurate and causes poor chromatography such as retention time shifts, late, early, no peaks or merging peaks. This article provides a quick...
This Information Applies To: Agilent 1290 Infinity (G4226A) and Agilent 1260 Infinity (G1367E) HiP Autosampler
Issue
Replacing the Needle Seat of the Autosampler is required when:
The needle seat is visibly damaged.
Leaks or blockages in the...
This Information Applies To: Agilent OpenLab CDS
Issue
This article discusses different types of bracketing modes and how to set up a bracketed calibration in Data Analysis for OpenLab CDS 2.x
Background
Occasionally, the instrument conditions...