Hi, Using one and the same column for different applications is possible, as long both solvents are permitted (column user manual e.g.).
The column should always be flushed very slowly from solvent A to B (0.1 mL/min). At least 5 times the column volume…
Hi Marty,
Thanks for taking the time to respond. I saved the data to a file and then printed the file and it was properly formatted. I think that there is an issue with printing to paper from the method. Since this is a long unsupported CS revision…
Hi Abbey
Thanks for the info!
So far we have only been running this on various forms of olive oil. But the waves in the baseline remain regardless of MOAH contant. I have run samples that were analyzed by outside labs (with COA) that contain 0ppm…
From a quick google search, it appears that hydroquinone may be detectable through LC/MS (ESI) in both positive and negative mode. It forms a protonated molecule ([M+H]+) at m/z 111 in positive mode and two ions at m/z 108 and 109 in negative mode.
…
奔跑的小短腿 , While an LC/MS system may operate in both positive and negative ion polarity modes, it will switch between the two polarities based on the user input in the acquisition software. It is not possible to acquire in both polarities simultaneously…
Hi Vincenzo,
Thank you for your answer. We have the CFR environment. WinUV 5.1.3.1042. (1042 indicates Part11).
I’ve looked for method files for the following apps; Advanced Reads which are .MAB, Concentration .MCN, Scan .MSW, and Simple Reads .MSR…
This Information Applies To: 5975 and 5977 GC/MS systems with MassHunter Acquisition software
Issue:
High-quality GC/MS data requires a low, and stable background of air and water present in the MS analyzer.
This article demonstrates a systematic...
This Information Applies To: Agilent 5975 and 5977 GC/MSD systems with MassHunter Acquisition software
Issue:
High-quality GC/MS data requires a low, and stable background of air and water present in the MS analyzer. This article describes how to...
This Information Applies To: Agilent 5977A/B GC/MSD with MassHunter GC/MS Acquisition Software
Issue:
This article describes the procedure to safely vent (shut down) the 5977A/B GC/MSD with Electron Ionization (EI)
The MSD will be damaged by incorrect...
This Information Applies To: HES ion source in 5977B/C GC/MSD, 7010 triple quadrupole GC/MS systems
Issue
The filaments are replaced as part of routine maintenance, or when they break (zero output current).
Items Required
Gloves, nylon,...
This Information Applies To: Agilent 5977A/B/C GC/MSD
Issue
The Electron Multiplier Horn must be replaced when it is contaminated or the EMV value reaches 3000 V with a clean ion source.
Items Required
Electron multiplier horn (5977B/C:...
This Information Applies To: Agilent 5973/ 5975/5977 GC/MSD with rotary vane foreline pump
Issue:
The oil mist filter is recommended to be replaced annually. If the color of the filter has changed or there is smell from the filter, the oil mist...
This Information Applies To: Agilent EI Source
Issue:
During normal operation of the GC/MS, the EI SST/Inert ion source will need to be disassembled to proceed with abrasive ion source cleaning and / or routine replacement of consumable parts (filaments...
This Information Applies To: Agilent G7129A/B/C Vialsampler
Issue:
Replacing the needle and needle seat of the Vialsampler is required when:
The needle is visibly damaged.
Leaks or blockages in the vialsampler are observed, especially between...
This Information Applies To: Agilent G1311A/B/C, G7111A/B, Quaternary Pumps, and G1310A/B, G7110B Isocratic Pumps, G1312A/B/C, G7112B Binary Pumps
Issue:
How to diagnose no flow or no pressure issues on binary, quaternary, or isocratic pumps.
...
This Information Applies To: Agilent UV and RID detectors
Issue
A clogged flow cell causes high backpressure. The clog can be removed by flushing the system with solvents at high pressure. If you suspect a clog or experience high backpressure due...