Sorry for the delayed response. I've found that either works fine. I typically try to keep injection volume low (0.2-1 uL for 1uM protein). I've mostly been using 50mM HEPES 150mM NaCl and get great deconvolution results, even with a 3 min short method…
I am getting message "draw command failed, EE34205" while running batch in G7129B infinity 2 autosampler. I have tried replacing transport assembly, but error is same. I have used another module G7129A Infinity autosampler with this LC, but same error…
Hello all! We are currently familiarising ourselves with the MassHunter data aquisition software (LC/MS Data Aquisition for 6200 series TOF/6500 series Q-TOF. Version B.08.00 Build 8.00.8058.3 SP1). We have been trying to find the most efficient way of…
How do you set up an auditory sound alert for when a method run completes? I was thinking it may have been a runtime event but I I can’t see my to find it .I set a alarm on my phone for the length of the experiment to remind myself to go check and set…
Lines 3-5 (blank, blank, 1/100ppb) make me think there is a contamination issue. Check the cps of Ni on those first 4 blanks. If you see a downward trend, that could be an indication of a Nickel contamination somewhere in the instrument that is washing…
For Research Use Only. Not for use in diagnostic procedures.
Here is a "best of" collection of links to manuals and tools for Agilent Automated Electrophoresis products (Bioanalyzer, TapeStation, Fragment Analyzer, ZAG DNA Analyzer, Femto Pulse, Oligo…
Hello,
My name is Ben Niemi from the New Hampshire Public Health Laboratories. We are currently running 6470 QQQ LC-MS/MS with a 1260 Infinity II LC Stack.
Recently we've encountered some condensation issues in our column compartment on the line that…
Recommendations:
Increase the Oven Temperature equilibration time from 0.5 minutes to 1 to 1.5 minutes. The GC oven walls need more time to cool down from 300° C back to 50° C to be ready for the next run. This affects run-to-run reproducibility.
…
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx analysis software
Issue
When a sample name starts with zero, the leading zero disappears from the exported data ( Figure 1 and Figure 2 ).
Background
Removing the leading...
This Information Applies To: Agilent 7890B GC system and 7697A headspace sampler
Issue
The 7697 Headspace Sampler can be configured to communicate directly with the 7890B GC. When this direct communication is configured, the 7697A headspace gets...
This Information Applies To: Agilent GC/MSD systems
Issue:
When performing MS tuning, there is error message "No Emission Current" and tune is aborted.
Steps to Follow
This error normally means that the selected filament is not working. Perform...
This Information Applies To: Agilent OpenLab CDS
Issue
Any desired processing method can be assigned to the current Result Set.
Requirements
A result set available to link the method to.
Correct Privilege (Role Membership) set up for the...
This Information Applies To: Agilent LC and CE Systems compatible with Lab Advisor
Issue:
Firmware is updated or downgraded when new features are added, or to fix some system bugs. It also solves compatibility issues when a new module is added to...
This Information Applies To: Agilent OpenLab CDS
Issue
This article shows how to export a report of peak area and retention time of a sequence in an Excel format.
Prerequisites
To be able to carry out the procedure as described, privileges...
This Information Applies To: Agilent OpenLab CDS
Issue
Reports in OpenLab CDS can be printed or saved manually in Data Analysys, or automatically during reprocessing, or acquisition.
Requirements
The user needs the following Roles and Privileges...
This Information Applies To: Agilent 5975 and 5977 Series GC/MSD systems.
Issue
During tuning, MassHunter prompted the error message: "MSD Fault- RFPA Difficulty", and tune action stopped.
Steps to Follow
Try to reconfigure the GC/MS....
This Information Applies To: Agilent OpenLab CSD 2.5 and above
Issue
The integration optimizer helps find a suitable set of integration events. It allows integration parameters to be tuned via sliders (peak width, slope sensitivity, height reject...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue:
This article explains how to install the Agilent 2100 Expert software and connect a PC to an Agilent 2100 Bioanalyzer system.
Steps to follow:
The latest version of...