Hi All, I need help to fix either baseline drifted down or change volume injection of water samples in GC/FID. Specifically, I am setting up a new GC instrument for analysis of methanol and alcohols in water samples with the following conditions: - Instrument…
Hello,
From the error code this look like error 5 with red light 2 and 4 on. Axis movement failed. Either something is blocking the X movement or there is a problem with the sensor. This will require repair. Please call the response center to get…
Hi all,
I have been trying to change our 8890 GC carrier gas with a FID from helium to nitrogen. To verify the change went well I first prepared a test mixture of lab solvents (methanol, acetone, pentane, dcm, isopropanol), ran it using He, then ran…
What likely happened is the wavelength check that is done after lamp ignition failed. When the lamp is switched on, the detector waits 1 minute to warm-up the lamp. Then a check of the deuterium emission line via the reference diode is performed. If the…
Hello pathagun ,
First thing you can double check is if any bubbles are present in the solvent lines between the solvent bottles and the degasser or the pump. You can also check if the solvent inlet filters in the bottles are blocked as these can…
Hi Sean...I think I've touched that particular instrument a long time ago. Gary - 50% 28 and 13% 32 after 24 hours is quite a big leak, maybe not massive but certainly needs fixing.
If the system worked fine before it was cooled off for a power fail…
Most likely the octopole PCA is failing. You could try dipping it, but if it happens again after redipping the board is failing and needs to be replaced.
It was on the equipment, you can check to be sure by running a performance check on the chiller. Our chiller passed all points on the performance check but failed on the ICP system.
thanks for your answer. Regarding the ion source (EI): it happens that in a relatively new column, the abundance of the peaks of the same control that I had been injecting almost 3 weeks ago, has decreased from 5 x10^5 to almost 1x10^4. I tried injecting…
I have tried two new lamp and its all fail intensity test on the controller. Then I borrow a standard flow cell from another LC and I got min. intensity pass but fail average intensity. So I use Methanol to clean the flow cell window and flush the flow…
This Information Applies To: Agilent 6820, 6890, 7820A GC systems with a Split/Splitless (SSL) inlet
Issue
Non-volatile sample matrix components will accumulate in the inlet liner and impact on analytical performance. The inlet liner should be changed...
This Information Applies To: Agilent headspace samplers (HS)
Issue
GC cycle time needs to be calculated correctly. Too short a GC cycle time will reduce sample quality and possibly stop the analysis prematurely. Too long GC cycle time can cause...
This Information Applies To: Agilent 7820A GC
Issue
7820A GC is designed with simplified keypad that comes with five buttons. They are Stop, Prep Run , Start, Up and Down arrow buttons. Changing the IP address requires a different method compared...
This Information Applies To: Agilent Single Needle Autosamplers
Background
Sometimes the user may observe liquid flowing from Port four on the autosampler injection valve. During an injection sequence, it is normal to have liquid from port four...
This Information Applies To: Agilent 4200, 4150, 2200 TapeStation systems
Issue
Can I run an assay using the ladder or sample buffer from a different assay if I have run out of the appropriate assay ladder or sample buffer? Is it possible to run...
This Information Applies To: Agilent ICP-MS 7700, 7800, 7850, 7900, 8800, and 8900 systems
Issue
Peristaltic pump tubing that has lost its elasticity will not deliver the sample to the nebulizer correctly. When the pump tubing appears limp or flattened...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx touch screen displays error code 90 after the Instrument Health Check (IHC), which happens when the AriaMx is switched on, or after running the onboard diagnostic tests...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx optical modules
Issue
AriaMx users can change which AriaMx optical modules are installed to upgrade the instrument capabilities or to troubleshoot.
Items Required
Any...
This Information Applies To: AriaMx real-time PCR system
Issue
Error 43 is displayed on the AriaMx touch screen. Error 43 is a voltage error and it normally indicates that the cartridge LED voltage or motor voltage being detected is abnormal. Sometimes...
This Information Applies To: Agilent 2100 Bioanalyzer system
Issue
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Background
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real...