Hi all :) For he experts here! I did my yearly maintenance (new gold seal, liner, column end cut to inlet and FID, new gas filters, ...). But since then, everytime there's a temperature change in the GC, the signal will show a sudden bump and then it…
I'm running a GC(7890A) / MS(5975C) with MassHunter Acquisition software. I automate much of my data analysis in Python by exporting the GC signal (FID) to .csv, and then exporting the MS signal using 'Export 3-D Data...' in MSD Productivity Chemstation…
Hello MikeL ,
Just wanted to post what we discussed during your call with the support center so if anyone else sees a similar problem they will have some things to try.
There can be issues with copying and pasting fairly large amounts of information…
Recently, we connected the column (CP7429) to TCD and FID using a Y splitter. However, there is no signal response on both TCD and FID after manually injection of the calibration gas (containing H2, CO, CH4, C2H4, C2H6 in CO2). The carrier gas is Ar.…
i had faced this troubleshooting before
it is probably a software problem.
first close masshunter and double click ( remove masshunter process)
open the soft again and try to create another method with same parameters it should work
also try to…
When performing the Eukariote Total RNA test on the same chip we had some samples with very degraded RNA and one sample with what seems like good quality RNA but the software failed to give a RIN value for that sample. Is there a way to still measure…
Dear Colleagues, apologizing for frequently wondering but I first faced this problem. GC produces a signal without turning on the FID Detector!? Please look at the attached photo. After turning on the detector and then conditioning the column while the…
For many FID methods, 10-20 Hz data rate is sufficient unless you're doing a fast method of some sort. You need enough data points to define the peak correctly and not get any spectral tilt. Typically 15-20 points across the peak is enough.
Hello AGILENT family,
i have a few questions on the analysis of Pb in blood samples.
am failing to get the right concentrations of the QC sample 8ppb instead it’s reading 0.08 ppb and at the same time it’s also showing overange (0.08o) even when my…
I am a user of an Agilent 7900 ICP-MS. It has been working fine since last month, but today (Oct 13, 2022) the software was not able to connect to the instrument. When I try to connect the MassHunter software to the ICPMS by going to settings > mainframe…
This Information Applies To: Agilent 6820, 6890, 7820A GC systems with a Split/Splitless (SSL) inlet
Issue
Non-volatile sample matrix components will accumulate in the inlet liner and impact on analytical performance. The inlet liner should be changed...
This Information Applies To: Agilent headspace samplers (HS)
Issue
GC cycle time needs to be calculated correctly. Too short a GC cycle time will reduce sample quality and possibly stop the analysis prematurely. Too long GC cycle time can cause...
This Information Applies To: Agilent 7820A GC
Issue
7820A GC is designed with simplified keypad that comes with five buttons. They are Stop, Prep Run , Start, Up and Down arrow buttons. Changing the IP address requires a different method compared...
This Information Applies To: Agilent Single Needle Autosamplers
Background
Sometimes the user may observe liquid flowing from Port four on the autosampler injection valve. During an injection sequence, it is normal to have liquid from port four...
This Information Applies To: Agilent 4200, 4150, 2200 TapeStation systems
Issue
Can I run an assay using the ladder or sample buffer from a different assay if I have run out of the appropriate assay ladder or sample buffer? Is it possible to run...
This Information Applies To: Agilent ICP-MS 7700, 7800, 7850, 7900, 8800, and 8900 systems
Issue
Peristaltic pump tubing that has lost its elasticity will not deliver the sample to the nebulizer correctly. When the pump tubing appears limp or flattened...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx touch screen displays error code 90 after the Instrument Health Check (IHC), which happens when the AriaMx is switched on, or after running the onboard diagnostic tests...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx optical modules
Issue
AriaMx users can change which AriaMx optical modules are installed to upgrade the instrument capabilities or to troubleshoot.
Items Required
Any...
This Information Applies To: AriaMx real-time PCR system
Issue
Error 43 is displayed on the AriaMx touch screen. Error 43 is a voltage error and it normally indicates that the cartridge LED voltage or motor voltage being detected is abnormal. Sometimes...
This Information Applies To: Agilent 2100 Bioanalyzer system
Issue
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Background
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real...