It all depends on your following steps. You can even use Methanol or Acetonitrile for the protein precipition: Protein Precipitation for Biological Fluid Samples Using Captiva EMR-Lipid
See also the chapter on protein precipitation in Agilent's Handbook…
The Digest of upcoming webinars has been updated as of April 30.
Did you miss April's GC Live Session Recording and Discussion Thread? Check it out here: ( +) Diagnostics From Your Desk, Troubleshooting From Your Kitchen Table - Follow-up Discussion…
Hello cjwwfd ,
B.06.00 is out of support at this point, but I can offer some general suggestions for current revisions and hopefully you can apply them to your version. Version 12.0 is the current release. If possible, I would investigate your options…
I'm experimenting the same error. Logbook displays <MSQQQ_1: QqQ fault detected: 1.23 vacuum system is not ready. This is typical during a pump down cycle or when the high vacuum pump is turned off during a vent cycle or fault condition>. Does anyone…
Hello DET ,
Sorry for the late response on this.
What sort of spiking do you see usually? What is the output of the FID, in pA?
Are you using any UPS systems, or are the GCs plugged directly into the wall?
Hi, I think it would be a good idea to upload your startup tune report in order to help you better.
Also I think you could take advantage of reading this post: https://community.agilent.com/technical/atomic-spec/f/forum/470/icp-ms-manual-tuning
Hello,
So, file based workstations store the full path to the methods in the sequences. If there is any change between the systems in the project path this is the expected behavior if you copy a sequence. If you open the sequence, browse out and find…
Hello guys,
I recently discovered, that it is possible to change your font properties in the injection list via shortcut (ctrl + b for "bold" and so on..).
Therefore I wanted to ask you, if there is some code for changing font types for CCF outputs…
Hi Mai,
Thank you for reaching out to Agilent technical support.
Please check:
if the data file you are trying to open was generated using TapeStation security module as that can be one reason for the file not to open and error out.
If anyone…
This Information Applies To: Agilent 6820, 6890, 7820A GC systems with a Split/Splitless (SSL) inlet
Issue
Non-volatile sample matrix components will accumulate in the inlet liner and impact on analytical performance. The inlet liner should be changed...
This Information Applies To: Agilent headspace samplers (HS)
Issue
GC cycle time needs to be calculated correctly. Too short a GC cycle time will reduce sample quality and possibly stop the analysis prematurely. Too long GC cycle time can cause...
This Information Applies To: Agilent 7820A GC
Issue
7820A GC is designed with simplified keypad that comes with five buttons. They are Stop, Prep Run , Start, Up and Down arrow buttons. Changing the IP address requires a different method compared...
This Information Applies To: Agilent Single Needle Autosamplers
Background
Sometimes the user may observe liquid flowing from Port four on the autosampler injection valve. During an injection sequence, it is normal to have liquid from port four...
This Information Applies To: Agilent 4200, 4150, 2200 TapeStation systems
Issue
Can I run an assay using the ladder or sample buffer from a different assay if I have run out of the appropriate assay ladder or sample buffer? Is it possible to run...
This Information Applies To: Agilent ICP-MS 7700, 7800, 7850, 7900, 8800, and 8900 systems
Issue
Peristaltic pump tubing that has lost its elasticity will not deliver the sample to the nebulizer correctly. When the pump tubing appears limp or flattened...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx touch screen displays error code 90 after the Instrument Health Check (IHC), which happens when the AriaMx is switched on, or after running the onboard diagnostic tests...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx optical modules
Issue
AriaMx users can change which AriaMx optical modules are installed to upgrade the instrument capabilities or to troubleshoot.
Items Required
Any...
This Information Applies To: AriaMx real-time PCR system
Issue
Error 43 is displayed on the AriaMx touch screen. Error 43 is a voltage error and it normally indicates that the cartridge LED voltage or motor voltage being detected is abnormal. Sometimes...
This Information Applies To: Agilent 2100 Bioanalyzer system
Issue
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Background
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real...