You can try to run evacuation mode to remove any liquid: Troubleshooting "Vacuum Limit Not Reached" Error in Agilent LC Pumps - Articles - LC Portal - Agilent Community
Hello Sharon, The System Verification tests reports display a test summary table and details for each test as table. It doesn't display graph and it cannot be modified. For the other applications (Scan, Concentration, Kinetic, and Thermal) the report…
Hi, could you please check if you have "Method Development" option activated in HS method settings? If you do, please set it to "None", save the method and try if it works. Thanks.
Hello sao.uch ,
There is not a way to do something like a spectral library match with chromatograms. If there are specific peaks you are interested in you could set up a simple quant method and define one run as your standard with a concentration of…
Here are some other helpful resources offered by Agilent:
Information on the 'Agilent Value Promise'
https://www.agilent.com/cs/library/brochures/brochure-agilent-value-promise-5991-1491en-agilent.pdf
Instrument lifetime information as it relates…
The GC is telling us that the front inlet pressure is 'Not Ready' is there a chance there is a leak in the inlet somewhere after the maintenance was performed?
Can the inlet hold pressure? If it can't hold pressure or does have a leak it will stop the…
Hello cpostigo ,
This error will occur if the batch has not been analyzed and there is no calibration curve available for your targets. The entries do appear to be accepted, and the calculation will be performed once the batch has been fully analyzed…
Hi, DP stands for Drug Product (I need to determine EtOH in a drug product not in a drug substance DS).
I'm loading 3 mL of sample in vial size 20 mL. The concentration of EtOH 4 mg/mL.
Yes of course, three replicates of three separate vials (same…
This Information Applies To: Agilent 6820, 6890, 7820A GC systems with a Split/Splitless (SSL) inlet
Issue
Non-volatile sample matrix components will accumulate in the inlet liner and impact on analytical performance. The inlet liner should be changed...
This Information Applies To: Agilent headspace samplers (HS)
Issue
GC cycle time needs to be calculated correctly. Too short a GC cycle time will reduce sample quality and possibly stop the analysis prematurely. Too long GC cycle time can cause...
This Information Applies To: Agilent 7820A GC
Issue
7820A GC is designed with simplified keypad that comes with five buttons. They are Stop, Prep Run , Start, Up and Down arrow buttons. Changing the IP address requires a different method compared...
This Information Applies To: Agilent Single Needle Autosamplers
Background
Sometimes the user may observe liquid flowing from Port four on the autosampler injection valve. During an injection sequence, it is normal to have liquid from port four...
This Information Applies To: Agilent 4200, 4150, 2200 TapeStation systems
Issue
Can I run an assay using the ladder or sample buffer from a different assay if I have run out of the appropriate assay ladder or sample buffer? Is it possible to run...
This Information Applies To: Agilent ICP-MS 7700, 7800, 7850, 7900, 8800, and 8900 systems
Issue
Peristaltic pump tubing that has lost its elasticity will not deliver the sample to the nebulizer correctly. When the pump tubing appears limp or flattened...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx touch screen displays error code 90 after the Instrument Health Check (IHC), which happens when the AriaMx is switched on, or after running the onboard diagnostic tests...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx optical modules
Issue
AriaMx users can change which AriaMx optical modules are installed to upgrade the instrument capabilities or to troubleshoot.
Items Required
Any...
This Information Applies To: AriaMx real-time PCR system
Issue
Error 43 is displayed on the AriaMx touch screen. Error 43 is a voltage error and it normally indicates that the cartridge LED voltage or motor voltage being detected is abnormal. Sometimes...
This Information Applies To: Agilent 2100 Bioanalyzer system
Issue
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Background
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real...