Hello, H2 is the fuel for the flame in the FID, so if you are out of H2 then the FID will not be able to light. That is very likely the reason why you are not seeing a signal from the FID.
Disconnect the sample probe from the valve and start the leak and restriction test. If it passes this step, it means that there is some restriction in the sample probe, enough to cause that part of the test to fail. Try a brand new probe. If it fails…
Seems then to indicate something in the flow path when including the mixer and damper. First, make sure that you do an elasticity calibration because you have changed important parts in the flow path. Then re-run the pump leak rate test with the mixer…
Hello experts!! I have 6460 LCMSMS system. It unfortunately failed in tuning even in brand new tuning solution. Auto tune repeatedly failed in peak width adjustment in MS1. What could be the problems and possible answers? Cleaning is done up to ion transfer…
No, the flows on the two columns downstream of the switch have to be equal. They are feed by the same pressure source, so since MSD is under vacuum and the FID is ambient, the column connected to the MSD has to have more restriction, to keep the flows…
I have decades of experience with consumer products, all on Agilent GC, GCMS, and HPLC.
If I was needing to quantitate 1,2-octanediol in cosmetics samples, I'd extract the sample with dimethylformamide and add BSTFA to make trimethylsilyl derivative…
I'm trying to create a report with a column which calculates the percentage of all integrated peaks above a specific threshold. I have created a report parameter to format the number of significant digits, this is a request of some of our clients. At…
I found the issue. I have "Hide duplicates" selected which prevents any additional Fails from being displayed. However, if I uncheck that box, there is a Fail in every row. This is a summary result, is there a way to make it display only once per group…
This Information Applies To: Agilent 4200 TapeStation 4150 TapeStation, TapeStation controller software
Issue
The system verification can be run to check the TapeStation performance, for example, when you suspect an instrument hardware fault.
...
This Information Applies To: Agilent 4200 and 4150 TapeStation software.
Issue
How to uninstall and reinstall the 4200 and 4150 TapeStation software.
Background
An uninstallation and reinstallation of the TapeStation software should be performed...
This Information Applies To: 2200 TapeStation instruments, 2200 TapeStation controller software
Issue:
The Agilent 2200 TapeStation controller software won't launch but displays the correct serial number in the top-right corner of the loading screen...
This Information Applies To: 4200, 4150 and 2200 TapeStation, TapeStation analysis software A02.02 or higher.
Issue:
When using the Agilent 4200, 4150 and 2200 TapeStation systems, the DV 200 of FFPE RNA samples can be calculated within the TapeStation...
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer, ProSize Data Analysis Software
Issue
How to export a ladder as a size calibration file and import it into a different run in ProSize.
Background
...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
It is possible to set up the 2100 Expert software so that the files are automatically exported each time a chip run is complete.
Steps to Follow
To turn...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software
Issue:
How to identify and troubleshoot late migration of the ladder, marker, and sample peaks in data generated on the Agilent 2100 Bioanalyzer system.
Background
...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
If communication between the 2100 Bioanalyzer and the PC cannot be established, or if an intermittent communication loss occurs, follow the instructions in...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
Instructions on how to import from or export data into LIMS software.
Steps to Follow
Data import
When importing sample names and sample comments from...