I had notice that the air volume is set to 1ul which is the same as the sample volume (I’m assuming is the same as the injected volume). You could try to set the air volume to 0ul and observe if the injection is consistent after this.
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Hi Mike, Thank you, yes correct, here is our situation to clarify, we upgraded in order to retire the Win7 OS. We had methods on Agilent UV 8454 ChemStation B.05.02 that managers would create, reviewers would approve, and analyst could use the methods…
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Wow, your peaks are super close to each other! Anything minor will affect your chromatogram. Maybe you should search for a better method? Anyways, what impacts the most are flow (are you running a gradient method?), organic concentration (again, gradient…
You can calculate the pressure on the flow cell by disconnecting the inlet capillary in the VWD. So the pressure in the VWD is the system pressure MINUS the pressure without VWD
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