This is normal as PONA columns are apolar phases with thin films. For your light HC's < C5 either a thick film (ca. 5 µm) or another phase/column would help, but higher HC's cannot be done then (see also Lance's answer). It's either switching or backflushing…
Are you running different methods in different worklists, or are the worklists all running the same method? I assume the same method across worklists, but this is not clear in your question.
Does this change in pressure lead to a change in your quality…
Hi Danielchsny, Yes power spikes from unscheduled power interruptions can cause damage to sensitive electronic circuits. Another contributing factor is that when power is lost to the LC/MS running under high vacuum the rough pump will stop, causing a…
Hi Carol, There is always a trade-off between Scan speed, mass resolution and sensitivity in any quadrupole mass filter. So if you decrease the resolution and keep scan speeds the same you will naturally get an increase in sensitivity. Based on the trouble…
What are the inlet and column configurations, flow rates, pressures, and timing setpoints? The fastest way to share that is to copy/paste the contents of the file acqmeth.txt that lives underneath your specific Our_Run_Method_Name.M subdirectory in Windows…
Yes - it was a bit of a mess - but I was able to fix it. Thanks for the heads up. Not everything could be fixed in the computer portion of the method. I had to go into the GC configuration and fix it there.
Thank you!
This Information Applies To: Agilent OpenLab ChemStation Edition Software (C.01.xx)
Issue
When your Agilent OpenLab ChemStation Edition software fails to launch with errors " Login failed with the following message: Configuration system failed to...
This Information Applies To: Agilent OpenLab ChemStation Edition Software (C.01.xx)
Issue
Agilent OpenLab ChemStation Edition software fails to launch with error containing the words " Autostart Macro failed ”. This article will provide you some...
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer and Oligo Pro II instruments, and their respective controller software.
Issue Failed to build pressure error comes up shortly after starting a separation...
This Information Applies To: Agilent 6400 Series triple quadrupole LC/MS systems equipped with a gate valve.
Issue
Removing the Ion Transfer Capillary for maintenance on instruments with gate valve. The gate valve allows ion capillary removal and...
This Information Applies To: Agilent OpenLab ChemStation, Agilent OpenLab CDS, Agilent OpenLab EZChrom
Issue
Due to the license error “ An error occurred connecting to the license server: The desired vendor daemon is down. Check the Imgrd log file...
This Information Applies To: HPLC Advisor app
Issue
Legacy HPLC method needs to be translated to a new column or LC system (HPLC to UHPLC or Analytical to Preparative LC).
Requirements
Install the most recent version of the HPLC Advisor...
This Information Applies To: Agilent 7890 A/B, 8860 and 8890 GC Systems with Split/Splitless (SSL), Multimode (MMI) or Programmable Temperature Vaporization (PTV) Inlets
Issue:
The Inlet Leak & Restriction Test is executed after every inlet maintenance...
This Information Applies To: 5000 series ICP-OES (5100,5110,5800,5900), AVS 4/6/7, ICP Expert version 7.X
Issue
The AVS can contribute to instrument performance issues such as sensitivity and precision. During troubleshooting, bypassing (or skipping...
This Information Applies To: Agilent GC Systems
Issue
Loss of peak resolution can be a combination of decrease in separation and increased peak width. Loss of resolution can impact on analytical reproducibility and accuracy.
Other chromatographic...
This Information Applies To: Agilent GC Systems
Issue
Contamination that creates ghost and carry over peaks can be introduced at different points including sample preparation, introduction, and separation. The additional peaks can cause false positive...