Hello, H2 is the fuel for the flame in the FID, so if you are out of H2 then the FID will not be able to light. That is very likely the reason why you are not seeing a signal from the FID.
Seems then to indicate something in the flow path when including the mixer and damper. First, make sure that you do an elasticity calibration because you have changed important parts in the flow path. Then re-run the pump leak rate test with the mixer…
Disconnect the sample probe from the valve and start the leak and restriction test. If it passes this step, it means that there is some restriction in the sample probe, enough to cause that part of the test to fail. Try a brand new probe. If it fails…
Hello experts!! I have 6460 LCMSMS system. It unfortunately failed in tuning even in brand new tuning solution. Auto tune repeatedly failed in peak width adjustment in MS1. What could be the problems and possible answers? Cleaning is done up to ion transfer…
No, the flows on the two columns downstream of the switch have to be equal. They are feed by the same pressure source, so since MSD is under vacuum and the FID is ambient, the column connected to the MSD has to have more restriction, to keep the flows…
I have decades of experience with consumer products, all on Agilent GC, GCMS, and HPLC.
If I was needing to quantitate 1,2-octanediol in cosmetics samples, I'd extract the sample with dimethylformamide and add BSTFA to make trimethylsilyl derivative…
I'm trying to create a report with a column which calculates the percentage of all integrated peaks above a specific threshold. I have created a report parameter to format the number of significant digits, this is a request of some of our clients. At…
I found the issue. I have "Hide duplicates" selected which prevents any additional Fails from being displayed. However, if I uncheck that box, there is a Fail in every row. This is a summary result, is there a way to make it display only once per group…
Hello everyone,
We have a problem with the acquisition methods on GC, but not on HPLC.
When we want to change an acquisition method that is in "approved" status to "generic" status in order to modify it, it is impossible to do so because the box is…
This Information Applies To: Agilent Liquid Chromatography (LC) systems
Issue:
Shutting down the HPLC for a long period requires following this general procedure to prevent damage to the system and the column.
Steps to follow:
Store the...
This Information Applies To: Agilent G7129A/B Autosampler with Sample Thermostat/Cooler G7167-60101 or G7167-60005.
Issue:
The autosampler may stop working from time to time, potentially giving off loud noises and switching off the light. The only...
This Information Applies To: Agilent 5977A/B GC/MSD with EI source, EI Extractor Source, or CI Source.
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte...
This Information Applies To: Agilent HES source, Agilent 5977 Series GC/MSD
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte response not improving...
This Information Applies To: Agilent 5977 and 5975 Series GC/MSD systems
Issue
The autotune fails and reports the error Tune action stopped: Gain and offset = 0 and peaks are too narrow!
Resolution
The MSD autotune program is run after routine...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
Condition new or cleaned ICP-MS skimmer cones and sampler cones by aspirating a Conditioning Solution before use.
Background
Drift up can be a sign of...
Turn On the GC/MS Interface and Oven Using OpenLab CDS
Multiple Results
This Information Applies To: Agilent GC/MS instruments controlled by OpenLab CDS software
Issue
Turning on the GC/MS interface heater and Oven using OpenLab CDS software.
Steps to Follow
From the Home tab, click Method > Instrument Setup...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900)
Issue
The signal monitor function in MassHunter can help with troubleshooting low sensitivity, carry over, contamination, or high background. It can also be used to optimize...
This Information Applies To: Agilent UV detectors (Variable Wavelength Detectors, Multiple Wavelength Detectors, and Diode Array Detectors)
Issue
Checking accumulated hours on the UV lamp.
Steps to Follow
There are four ways to check the accumulated...
This Information Applies To: Agilent 7700/7800/7850/7900/8800/8900 ICP-MS
Issue:
Poor internal standard (ISTD) mixing can cause high relative standard deviation (RSD).
The recommendations in this article are applicable if:
It takes a long...