I am building a report for some GC-FID data from a GC-MS/FID instrument. I have managed to build a report that contains the chromatogram and the table of peaks. I can scale the X-axis to the scale i need but I have been unable to scale the y-axis to show…
I am using OpenLab Chemstation C.01.10 on an 8890 with dual FIDs. I was trained on HP's version of Chemstation that reported both detector quant amounts in one row for precision purposes. It made it 10x easier to review data and confirm sample concentrations…
Varian 710 oes, nebulization is ok, fog in the chamber is omogeneous, tubing, nebulizer, torch, cone cleaned and alligned.
Work fine till 2 weeks ago, after i've experienced this: fail to execute wavelenght calibration end torch allign, No response…
Hello Everyone,
I am using 7820A GC coupled with 5977B Mass Spec. After use I close the He tank and later on I get 2 messages - 'Front inlet flow shutdown' and 'Front inlet pressure shutdown'. I restarted the software but it fails to load any GC method…
Hello, we are setting up a seahorse assay for a immunology master class. We want to measure the glycolytic rate in human PBMCs or purified human CD4 T cells activated vs non activated. We fail to see any difference. Has anyone done this kind of assay…
We have a Duo 2.5 foreline pump that was replaced in June of this year (2023). The previous Duo 2.5 installed had a sudden catastrophic leak, thus the replacement in June. The oil was filled up halfway and I set a marker with tape to monitor the level…
Hi Juliana
Yes, most likely the capacitors and/or the RF Tube. However, you can try to reduce the run power to 1.1Kw in the default ignition table. You may then be able to run for a few weeks with this reduced power level. You will need to reduce the…
I have started using GC-FID (Agilent 8890) since March for the first time. We are trying to measure the methane. I was trying to make a standard surve, so I injected 1000 ppm methane standard at first. Just to let you guys know no samples has been run…
Hi vitiin96,
this is very generic and any book on GC troubleshooting or the videos on the Agilent website could help. For the 7890 there is a troubleshooting manual available.
Good practice in GC is when you get a (new) column, you have an own suitability…
Hi byoo,
Do you get any failed to communicate or any other message when you start up the software? You are correct if the busy light should be constantly flashing. This means you got a successfuk firmware load from the PC from ethernet connection. Can…
This Information Applies To: Agilent Liquid Chromatography (LC) systems
Issue:
Shutting down the HPLC for a long period requires following this general procedure to prevent damage to the system and the column.
Steps to follow:
Store the...
This Information Applies To: Agilent G7129A/B Autosampler with Sample Thermostat/Cooler G7167-60101 or G7167-60005.
Issue:
The autosampler may stop working from time to time, potentially giving off loud noises and switching off the light. The only...
This Information Applies To: Agilent 5977A/B GC/MSD with EI source, EI Extractor Source, or CI Source.
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte...
This Information Applies To: Agilent HES source, Agilent 5977 Series GC/MSD
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte response not improving...
This Information Applies To: Agilent 5977 and 5975 Series GC/MSD systems
Issue
The autotune fails and reports the error Tune action stopped: Gain and offset = 0 and peaks are too narrow!
Resolution
The MSD autotune program is run after routine...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
Condition new or cleaned ICP-MS skimmer cones and sampler cones by aspirating a Conditioning Solution before use.
Background
Drift up can be a sign of...
Turn On the GC/MS Interface and Oven Using OpenLab CDS
Multiple Results
This Information Applies To: Agilent GC/MS instruments controlled by OpenLab CDS software
Issue
Turning on the GC/MS interface heater and Oven using OpenLab CDS software.
Steps to Follow
From the Home tab, click Method > Instrument Setup...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900)
Issue
The signal monitor function in MassHunter can help with troubleshooting low sensitivity, carry over, contamination, or high background. It can also be used to optimize...
This Information Applies To: Agilent UV detectors (Variable Wavelength Detectors, Multiple Wavelength Detectors, and Diode Array Detectors)
Issue
Checking accumulated hours on the UV lamp.
Steps to Follow
There are four ways to check the accumulated...
This Information Applies To: Agilent 7700/7800/7850/7900/8800/8900 ICP-MS
Issue:
Poor internal standard (ISTD) mixing can cause high relative standard deviation (RSD).
The recommendations in this article are applicable if:
It takes a long...