This Information Applies To: Agilent 6400 Series triple quadrupole LC/MS systems
Autotune fails to complete.
Agilent 6400 LC/MS systems use an Autotune algorithm to optimize the sensitivity and mass resolution of the instrument. Sometimes when running the autotune procedure the autotune does not complete and the user is given an Autotune failed message in MassHunter. One of the main reasons for Autotune failure is a lack of sensitivity or abundance of the tuning ions. This article goes through some simple checks that a user can do to try to get the instrument to pass Autotune.
Resolution / Steps to Follow
|Learn how to effectively tune and calibrate your Agilent 6400 series LC-QQQ instrument:
LCMS-6400-1120sV2 - Tune and Calibrate Agilent 6400 Series Triple Quadrupole LC/MS with MassHunter Acquisition (Ver. 10.0) e-learning course available from Agilent education