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  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  LC/MS Systems
  • Component :  LC/MS Single Quadrupole
  • Product Name :  6110 Single Quadrupole LC/MS 6120 Single Quadrupole LC/MS 6130 Single Quadrupole LC/MS 6140 Single Quadrupole LC/MS 6150 Single Quadrupole LC/MS InfinityLab LC/MSD InfinityLab LC/MSD XT InfinityLab LC/MSD iQ
  • Task :  Operation
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How to Pump Down your Single Quadrupole LC/MS System Using OpenLab CDS

Answer

This Information Applies To: 6100 Series single quadrupole LC/MS systems

Issue

Instructions for starting and pumping down your Agilent Single Quad 6100 series LC/MS instrument using OpenLab CDS software.

Requirements

This procedure starts with the MS vented and powered off.

Steps to Follow

  1. Make sure that the MS power cord is plugged into a grounded electrical outlet.

  2. Turn on the power to the instrument using the power switch on the bottom-left corner of the instrument (see highlighted section in Figure 1).

  3. Wait for a couple of minutes for the smartcard in the MS to start before communication between the MS and OpenLab is possible. You should hear the Calibrant Delivery System (CDS) valve switch position approximately one to three minutes after turning on the MS. This sound indicates that the MS smartcard has initialized and the MS is ready for communication and OpenLab control.


    Figure 1
    Figure 1. Power button location (bottom-left).

  4. Turn on each LC module while waiting for the MS Smartcard to initialize.

  5. Start the OpenLab Acquisition program.

  6. If not already visible, in the Home tab f the ribbon, click Instrument Status. (See Figure 2)


    Figure 2
    Figure 2. Instrument Status button location.

  7. If necessary, click Take to acquire control of the instrument. In Figure 3, Take is already selected.

    Figure 3
    Figure 3. Take instrument.

  8. In the Instrument Status window, click the Dashboard tab.

  9. Right-click the SQ device panel and click Pump Down.

      
    Caution: Tuning or trying to start analysis on an MS that is not sufficiently pumped down and equilibrated to temperature will result poor system performance, and the potential for instrument contamination and damage to the ion optics electronics. Do not rush the next step.

  10. Wait for your MS to complete the pump down process and reach your normal operating pressures.

    1. The rough vacuum should be between 1.0 - 2.5 Torr with a 0.5 mm I.D Ion capillary installed, or between 2.0 - 3.5 Torr with 0.6 mm I.D Ion capillary installed.

    2. The high vacuum should be 3.0 x 10-6 - 2.0 x 10-5 Torr

    3. Turbo speed from 97 to 103%

  11. Once the MS is sufficiently pumped down, load your required analytical method, and wait for your temperature and gas flow setpoints to be reached and heated zones to equilibrate.

      
    Caution: Wait at least 2 hours for all MS heated zones to fully equilibrate after reaching temperature setpoints before tuning your instrument to ensure a stable tune result.

  12. When ready for analysis, the SQ panel in the Instrument Status will turn green.

    Figure 4 
    Figure 4. SQ device status Ready.

  13. It is recommended after pumping down and allowing the MS to equilibrate, to run a tune on the instrument to check system performance before analyzing samples. If you have any issue with the pump down and start of your LC/MS instrument, then please contact your local Agilent Service representative.

 

  Learn how to effectively operate your Agilent Single Quadrupole LC/MS:
Agilent Single Quadrupole LC/MSD with OpenLab CDS e-learning path available from Agilent education
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