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Articles Agilent Fragment Analyzer Message: Setup Incorrect - Ladder File does not Exist
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  • Created :  10 Nov 2022
  • Modified :  7 Feb 2023
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Software Automated Electrophoresis Systems
  • Component :  Fragment Analyzer Systems
  • Product Name :  5200 Fragment Analyzer System 5300 Fragment Analyzer System 5400 Fragment Analyzer System Fragment Analyzer controller software
  • Task :  Data Acquisition Troubleshooting
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Agilent Fragment Analyzer Message: Setup Incorrect - Ladder File does not Exist

Answer

This Information Applies To: 5200/5300/5400 Fragment Analyzer, Fragment Analyzer controller software


Issue:

When trying to run a method, the Agilent Fragment Analyzer controller software shows the message Setup incorrect - Ladder file does not exist (see Figure 1).

  
Figure 1: Setup Incorrect - Ladder file does not exist


Resolution: 

The software displays the error message because the check box Use size calibration file is selected (marked in Figure 1), which requires the user to load a .SCAL ladder file. If the Size calibration file path section is left blank, this error message is displayed. 

If it is the first time you are running this method, you will need to run a ladder in the sample plate. For this initial run, select Create size calibration file in the Separation Setup dialog box and a .SCAL file will be created when the run completes. This .SCAL file can be used for future runs with the same method. Alternatively, you may export a ladder file from an existing run through ProSize (open the run and click Analysis > Show size calibration > Export). 

For future runs, you wouldn’t need to run a ladder and can select the Use size calibration file check box. Then load the saved .SCAL file by clicking the three dots behind the Size calibration file path section (see Figure 1), which will be used for analysis of the run in ProSize. 

 

 Caution
Caution: Use the saved ladder for no longer than one week. The runs you are importing the saved ladder into should be run with reagents from the same vials.

 

Tip
Learn how to effectively maintain your Agilent Fragment Analyzer:
5200, 5300, and 5400 Fragment Analyzer System Manual user manual on Agilent.com

For Research Use Only. Not for use in diagnostic procedures.

PR7000-8226

 
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