This Information Applies To: 5200/5300/5400 Fragment Analyzer, Fragment Analyzer controller software
Over time the dynamic coating polymer on the capillaries needs to be refreshed. Failure to do so can lead to a loss of performance. Indicators that the dynamic coating needs refreshing include:
Also, if capillaries are stored dry or the Storage Solution is not replaced they can clog. Indicators of clogged capillaries include no flow through the capillaries leading to no signal. Flushing the capillaries with sodium hydroxide (NaOH) followed by Conditioning Solution rejuvenates the dynamic coating and unclogs capillaries restoring instrument performance.
|Recommended maintenance schedule: Agilent recommends performing the NaOH flush as part of troubleshooting procedures and your routine maintenance to restore separation performance. You can add it as part of a regular cleaning schedule weekly or biweekly to ensure optimal performance.|
Steps to follow:
|Tips: Further information on the NaOH flush procedure is available in the Fragment Analyzer System Manual Appendix. To remove dried gel or Storage Solution, consider performing Method B before the NaOH flush: submerse capillary ends and electrodes in hot water (65–93 °C). Sometimes, the reservoir vent valve needs to be cleaned as well (for more details, see here).|
|Warning: Sodium hydroxide is corrosive and can cause severe burns when it contacts skin. Always wear appropriate personal protective equipment when handling sodium hydroxide.|
|≥12 mL||for a 12-capillary array|
|≥25 mL||for a 48-capillary array|
|≥50 mL||for a 96-capillary array|
In the main screen Operation tab > Capillary Array - Conditioning click Add to queue
From the Method drop down menu, select Method C Flush - 0.5 N NaOH - 10min 200psi.mthdc (you can check the parameters by clicking Edit).
Select OK and run the method from the queue (see Figure 1)
Caution: If NaOH is not washed out of the capillaries immediately after the NaOH flush, it can damage the capillary array.
Figure 2. Where to find the Full Conditioning method
|Learn how to effectively maintain your Agilent Fragment Analyzer:|
For Research Use Only. Not for use in diagnostic procedures.