Infinity 1260 II vialsampler standards dilution calibration programm

Hi,

there is no a downloadable program at the moment. But you could take a look at the Application Note Let Your Autosampler Do Your Pipetting (agilent.com). It describes the sampler programming approach to create the dilution series for calibration purposes.

Hi thanks for your response, but  there s no sequence example in this application note and description of the different functions in autosampler manual are very simple.
Is there any other manual?

regards.

L Stephan

Hi,
Thanks i m not at the lab this week but i will try to change line 5.
I tried to modify both volumes in order to have a maximum added volumes less than 100µL ( like 10 from stock solution and 60 diluent volvent) but i had the same error.

I will tell you as soon as i ll test that.

Have a nice day.

Fingers crossed 

Hi Nadjan,

I continue my tests ;-)
So for the moment the injector program seems to work now. I weighed my vials several times and i ve got my 100µl as well in vial after dilution by five 80 + 20 µL. I saw the bubbles from the loop to shake my solutions.

In fact, the problem perharps comes from my sequence in wich i m injecting a blank from a vial in position P1-B1 as you can see here 


my injector program working :

Do you have an idea if there s a problem with my sequence test?

Thanks a lot.

L Stephan

Lstephan 

Are you still getting an error with this sequence?  What error message do you see?

/Andy

Hi Andy,
Yes i m still getting an error this one:

I think that the error is coming from the sequence.
The autosampler is taking the vial P1-B1, and after the needle is coming into vial the error appears.

L Stephan 

Hi Lstephan 

Looking further down the list of errors I can see a "allowed volume exceeded".  I wonder what other errors are below this.  This is one where it would be good to be able to watch the sampler to see which of the steps in your injector program the error happens. I think the problem is in the injector program, not the sequence.  You could change the vial in the sequence to match the first vial in the injector program to see if that makes a difference.

The other thing that I would check is that, when you edit the whole method from "Instrument...Set up Instrument Method" to make sure that the injector program is actually enabled:

/Andy

__PRESENT__PRESENT

Hi Lstephan 

Looking further down the list of errors I can see a "allowed volume exceeded".  I wonder what other errors are below this.  This is one where it would be good to be able to watch the sampler to see which of the steps in your injector program the error happens. I think the problem is in the injector program, not the sequence.  You could change the vial in the sequence to match the first vial in the injector program to see if that makes a difference.

The other thing that I would check is that, when you edit the whole method from "Instrument...Set up Instrument Method" to make sure that the injector program is actually enabled:

/Andy

__PRESENT__PRESENT

Hi Andy thanks a lot for your response.

Something is very strange,i made the injector program as you can see below 



I made this to prepare 2 levels of dilution.
The first dilution is ok but the injector took the vial P1-B1 specified in the sequence line instead making the second dilution as specified in the injector program.

the sequence


Any idea?

Not very simple to use the dilution function :-)

Hi Lstephan,

in fact, sounds interesting... When you specify the sample vial - the sample will be taken only if in the injector program you say "Draw from sample". I can see that you have this line in the recent injector program. Otherwise, if there is no line "Draw from sample", the location (in this example, P1-B1) will be ignored. Maybe you even want it? I am thinking that you could benefit from having one method with an injector program and using it to prepare dilutions. And the second method you can use for the actual analytics, without the injector program being activated. It is very strange that for washing the needle goes to P1-B1. Could you please check what is configured in the vialsampler as the washing step, a wash vial or flush port? Could it be that the wash vial is also assigned to P1-B1? Strange that the first needle wash works well and on the second the needle "wanders" somewhere else.

It also seems to me that you should remove line 9 and line 20 "Draw default volume from sample". The program draws 2 times from the sample by 5µL(?) and ejects it into P1-A6 in the first cycle and P1-A5 in the second cycle (part of the repeat 3 times cycle). It is more logical if the needle goes to P1-B1 earlier than you highlighted on the screenshot. Draw from sample command is one before the wash the needle step.

Many thanks Nadjan for all that.
I will try it :-)

Ludovic

Hi Nadja and Andy,

That’s one small step for mankind, one giant step for me :-)


I finally succeeded in my dilutions five levels with the injector program !
I m testing the linearity of the calibration.

Many thanks for your help :-) !

Ludovic

Good luck and all the best!