Infinity 1260 II vialsampler standards dilution calibration programm

Hi,

there is no a downloadable program at the moment. But you could take a look at the Application Note Let Your Autosampler Do Your Pipetting (agilent.com). It describes the sampler programming approach to create the dilution series for calibration purposes.

Hi thanks for your response, but  there s no sequence example in this application note and description of the different functions in autosampler manual are very simple.
Is there any other manual?

regards.

L Stephan

Hi Andy thanks a lot for your response.

Something is very strange,i made the injector program as you can see below 



I made this to prepare 2 levels of dilution.
The first dilution is ok but the injector took the vial P1-B1 specified in the sequence line instead making the second dilution as specified in the injector program.

the sequence


Any idea?

Not very simple to use the dilution function :-)

Hi Lstephan,

in fact, sounds interesting... When you specify the sample vial - the sample will be taken only if in the injector program you say "Draw from sample". I can see that you have this line in the recent injector program. Otherwise, if there is no line "Draw from sample", the location (in this example, P1-B1) will be ignored. Maybe you even want it? I am thinking that you could benefit from having one method with an injector program and using it to prepare dilutions. And the second method you can use for the actual analytics, without the injector program being activated. It is very strange that for washing the needle goes to P1-B1. Could you please check what is configured in the vialsampler as the washing step, a wash vial or flush port? Could it be that the wash vial is also assigned to P1-B1? Strange that the first needle wash works well and on the second the needle "wanders" somewhere else.

It also seems to me that you should remove line 9 and line 20 "Draw default volume from sample". The program draws 2 times from the sample by 5µL(?) and ejects it into P1-A6 in the first cycle and P1-A5 in the second cycle (part of the repeat 3 times cycle). It is more logical if the needle goes to P1-B1 earlier than you highlighted on the screenshot. Draw from sample command is one before the wash the needle step.

Many thanks Nadjan for all that.
I will try it :-)

Ludovic

Hi Nadja and Andy,

That’s one small step for mankind, one giant step for me :-)


I finally succeeded in my dilutions five levels with the injector program !
I m testing the linearity of the calibration.

Many thanks for your help :-) !

Ludovic

Good luck and all the best!

Good luck and all the best!