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    • When Should Liquid be Observed from Port Four on LC Autosamplers?

      This Information Applies To: Agilent Single Needle Autosamplers Background Sometimes the user may observe liquid flowing from Port four on the autosampler injection valve. During an injection sequence, it is normal to have liquid from port four. This port is used to expel the solvent when the Analytical...

    • Continuous Air Bubbles Observed in Waste Tubing on Manual Purge Valve

      This Information Applies To: Agilent LC pumps with manual purge valve. Issue A good practice before using a system is to purge the system to remove air. This is done by observing the clear tubing from the solvent bottle as well as the waste line from the purge valve, until no air bubbles are noticed...

    • Determining if a Communication Issue is Due to an LC Module Hardware Failure

      This Information Applies To: Agilent Analytical HPLC Modules Issues Verifying that the nature of a Communication Issue is caused by an LC Module Failure. Resolution When a user launches the Chromatography Data System (CDS) and it connects to the LC system, all icons will appear green once turned...

    • Inhibit Baseline Autozero in Agilent Fluorescence Detectors

      This Information Applies To: Agilent Fluorescence Detectors (FLD) Issue Sometimes it’s necessary to change FLD EX and EM wavelengths during a run since not all components will excite or emit at the same wavelengths. However, when the wavelength change is made, the detector will autozero which will...

    • Understanding Pressures within an HPLC System

      This Information Applies To: Agilent Analytical Scale LC Systems Issue To troubleshoot high backpressure within an HPLC system, it’s necessary to understand the expected pressure throughout an operating system. The total pressure for an LC is measured by a pressure sensor in the pump. In the hypothetical...
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