Hi, the peak area has no influence on the calculation of Mw when measuring with concentration detectors only (RI, UV, ELSD e.g.). When performing Viscometry & Lightscattering measurements, the peak area is needed for the calculation. As an additional…
Occasionally the tune files get corrupted and you need to go back to an old one or start again from the default atune.u file. Glad the problem is resolved!
Part 1 included many questions and some answers about the maximum abundance that should be detected at the electron multiplier. Another important consideration is how much sample should go into the column.
See Which Electron Ionization Ion Source? Part…
Thank you for taking a moment to review the Community Guidelines. You agree to all of the guidelines posted and linked here when using the Agilent Community. These guidelines are here to help you be a valuable contributor to the community and make sure…
Hi, I have an old 6890 with a jet direct card. We lost power to the instrument and since then it has not been able to connect to the PC. I tried updating the bootp software with the correct mac address and power cycled instrument with the same issue.…
Support and educational resources for the Microplate Centrifuge and Automated Centrifuge Loader .
Automation Solutions Knowledge Base
Microplate-Handling Device Guides
PDF user guides
Support Documents
G5582A Centrifuge Installation Checklist…
-Have you tried a diferent column ? An old column could have retention time issues and peak distortion.. -Have you had any pressure fluctuations during your run ? Try running a Pump leak rate test, to see if the problem is not in the pump-head. -Do a…
This Information Applies To: All Agilent 6000 Series LC/MS Systems without a gate valve for ion capillary installation/removal. For instructions for removing an ion capillary on LC/MS instruments with a gate valve installed, please click here
Issue...
This Information Applies To: Agilent 6000 series LC/MS instruments
Issue:
Cleaning the ion transfer capillary as part of regular maintenance or as part of troubleshooting when a loss of sensitivity in the instrument occurs.
Steps to follow ...
This Information Applies To: Agilent 6000 Series LC/MS systems.
Issue
Efficient electrospray ionization (ESI) in your LC/MS ion source requires a properly adjusted nebulizer needle in good condition. The nebulizer needle is a consumable item in...
This Information Applies To: Agilent 6000 Series LC/MS systems.
Issue
Checking and adjusting the nebulizer needle is part of regular maintenance or troubleshooting tasks. It is suggested when there is an issue with nebulizer spray shape or spray...
This Information Applies To: Agilent Single Quadrupole LC/MS systems controlled by OpenLab CDS software
Issue
How to check and recognize the current Instrument Status of your 6100 Series and InfinityLab LC/MS systems using OpenLab CDS software....
This Information Applies To: Agilent LC/MS Systems
Issue
Contamination of the tune mix or the Calibrant Delivery System (CDS) of your LC/MS system can cause Autotune failures and drops in sensitivity due to ion suppression.
Items Required...
This Information Applies To: Agilent LC/MS Systems
Issue
When there is suspected contamination of the CDS, low tune response, or possible blockage in the CDS, flushing the Calibrant system may be required.
Warning : This procedure risks...
This Information Applies To: Agilent LC/MS System
Issue
Instructions on how to check the level of calibrant in your LC/MS system, and refill the calibrant bottle if necessary.
Requirements
Checking the level of calibrant should be performed...
This Information Applies To: Agilent 6100 Series LC/MS Instruments
Issue
Instructions on how to fill the calibrant bottle in Agilent 6100 Series LC/MS Instruments when calibrant levels are running low.
Background
The ability to autotune your...
This Information Applies To: Agilent 6000 Series LC/MS systems
Issue
The article details the correct tuning mix to use according to your LC/MS system and the source type installed on your system. In cases where Agilent tuning mixes need to be diluted...