• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  LC/MS Systems
  • Component :  LC/MS Quadrupole TOF LC/MS Single Quadrupole LC/MS Time of Flight LC/MS Triple Quadrupole
  • Product Name :  6110 Single Quadrupole LC/MS 6120 Single Quadrupole LC/MS 6130 Single Quadrupole LC/MS 6140 Single Quadrupole LC/MS 6150 Single Quadrupole LC/MS 6220A Time of Flight LC/MS 6224A Time of Flight LC/MS 6230 Time of Flight LC/MS 6410 Triple Quadripole LC/MS 6420 Triple Quadripole LC/MS 6430 Triple Quadripole LC/MS 6460 Triple Quadripole LC/MS 6470 Triple Quadripole LC/MS 6475 Triple Quadripole LC/MS 6490 Triple Quadripole LC/MS 6495 Triple Quadripole LC/MS 6510 Q-TOF LC/MS 6520 Q-TOF LC/MS 6530 Q-TOF LC/MS 6538 Q-TOF LC/MS 6540 LC/Q-TOF 6545 LC/Q-TOF 6545XT AdvanceBio LC/Q-TOF 6546 LC/Q-TOF 6550 iFunnel LC/Q-TOF 6560 Ion Mobility LC/Q-TOF InfinityLab LC/MSD InfinityLab LC/MSD XT InfinityLab LC/MSD iQ Ultivo Triple Quadrupole LC/MS
  • Task :  Operation
Related Articles

  • Filling the Calibrant Bottle in Agilent 6100 Series Single Quadrupole LC/MSD Systems

    This Information Applies To: Agilent 6100 Series LC/MS Instruments Issue Instructions on how to fill the calibrant bottle in Agilent 6100 Series LC/MS Instruments when calibrant levels are running low. Background The ability to autotune your Agilent LC/MS system relies on providing the instrument with the correct composition and concentration of calibration solution. This article provides detailed instructions on how to make up your required calibration solution and refill your calibrant...

  • Filling the Calibrant Bottle in 6200/6400/6500 Series LC/MS Systems

    This Information Applies To: Agilent 6200/6400/6500 Series LC/MS Systems Issue Instructions on how to fill the calibrant bottle on 6200/6400/6500 Series LC/MS Instruments when calibrant levels are running low. Background The ability to autotune your Agilent LC/MS system relies on providing the instrument with the correct composition and concentration of calibration solution. Items Required The type of Calibrant used for autotune depends on the type of Ion Source installed on your...

  • GC/MSD Autotune Stopped: Gain and Offset Error

    This Information Applies To: Agilent 5977 and 5975 Series GC/MSD systems Issue The autotune fails and reports the error Tune action stopped: Gain and offset = 0 and peaks are too narrow! Resolution The MSD autotune program is run after routine maintenance and at customer set intervals when analyzing samples. Possible causes of Tune action stopped error include: The instrument was not assembled correctly after a routine procedure. Check that all steps of the routine procedure were...

  • Refilling the EI Calibration Vial for the Agilent 5975 and 5977 Series GC/MSD

    This Information Applies To: Agilent 5975 and 5977 series GC/MSD Issue: Tuning the Agilent GC/MSD with EI sources, requires the use of perfluorotributylamine (PFTBA). The calibration vial will be depleted eventually due to regular use. This article explains how to refill the calibration vial. Steps to follow: Materials required GC/MS Tuning Standard - PFTBA (p/n 05971-60571 ) Syringe or pipette To Refill the Calibrant vial Stop any tuning or data acquisition. Turn...

  • Refilling the CI Calibrant Vial for the Agilent 5975 and 5977 Series GC/MSD

    This Information Applies To: Agilent 5975 and 5977 series GC/MSD Issue: If the PFDTD signal is absent or low, but the reagent ions are normal, you may have run out of CI Calibrant. Inspect the calibration vial on the back of the flow controller. If necessary, fill the vial with PFDTD according to the instructions in this article. Steps to Follow : Materials needed Wrench, open-end, 1/4-inch × 5/16-inch (p/n 8710–0510 ) PFDTD calibrant, for GC/MS – 1 mL (p/n 8500–8510 ) Syringe...

Checking the Calibrant Level in your Agilent LC/MS System

Answer

This Information Applies To: Agilent LC/MS System

Issue

Instructions on how to check the level of calibrant in your LC/MS system, and refill the calibrant bottle if necessary.

Requirements

Checking the level of calibrant should be performed weekly, or more often if frequent instrument tuning is required.

Steps to Follow

  1. Open the door or remove the cover at the front of the instrument to reveal the calibrant bottle
  2. Carefully check that the intake tube that draws up calibration solution into the CDS system is fully immersed in solution, see Figure 1.
      
    Tips: Figure 1 shows a Q-TOF system with both a calibrant bottle (right), and a bottle for reference mass solution (left). 6100 and 6400 series LC/MS instruments have one calibrant bottle.
  3. If the calibration solution is within a few millimeters of the end of intake tube, then refill the calibrant bottle. See the following articles for detailed instructions on this procedure.



Figure 1. How to check the level of calibrant in your LC/MS system

 

 
Was this helpful?
Privacy Statement
Terms of Use
Contact Us
Site Help