This Information Applies To: Agilent 6000 Series LC/MS systems
Issue
The article details the correct tuning mix to use according to your LC/MS system and the source type installed on your system. In cases where Agilent tuning mixes need to be diluted, detailed instructions are also provided.
Requirements
Tuning is the optimization of electrical elements in a system that affect ion transmission and peak shape using a mixture of known compounds. Tuning mix containers should be kept in a cool, well-ventilated area and have a shelf life of one year.
Items Required
Agilent has five different tuning mixes available depending on your application and source type:
Steps to Follow
Single Quadrupole Systems
Tuning mix used for single quadrupole 6100 and LC/MSD systems depends on model and ion source type.
| Model | Ion Source | ||||
|---|---|---|---|---|---|
| ESI | APCI | APPI | MM | AJS | |
| InfinityLab LC/MSD iQ | ESI-L Tuning Mix | NA | NA | NA | NA |
| InfinityLab LC/MSD | ESI Tuning Mix | APCI/APPI Tuning Mix | APCI/APPI Tuning Mix | APCI/APPI Tuning Mix | NA |
| InfinityLab LC/MSD XT | ESI Tuning Mix | APCI/APPI Tuning Mix | APCI/APPI Tuning Mix | APCI/APPI Tuning Mix | ESI-L Tuning Mix |
| 6110 | ESI Tuning Mix | APCI/APPI Tuning Mix | APCI/APPI Tuning Mix | APCI/APPI Tuning Mix | NA |
| 6120 | ESI Tuning Mix | APCI/APPI Tuning Mix | APCI/APPI Tuning Mix | APCI/APPI Tuning Mix | NA |
| 6130 | ESI Tuning Mix | APCI/APPI Tuning Mix | APCI/APPI Tuning Mix | APCI/APPI Tuning Mix | ESI-L Tuning Mix |
| 6140 | ESI-L Tuning Mix | APCI-L Tuning Mix | APCI/APPI Tuning Mix | ESI-L Tuning Mix | NA |
| 6150 | ESI-L Tuning Mix | APCI-L Tuning Mix | APCI/APPI Tuning Mix | ESI-L Tuning Mix | ESI-L Tuning Mix |
Table 1. Agilent LC/MSD Single Quadrupole tune mix matrix
Triple Quadrupole, TOF, and Q-TOF Systems
Tuning mix used for 6200, 6400, and 6500 systems depend on ion source type.
| Model | Ion Source | ||||
|---|---|---|---|---|---|
| ESI | APCI | APPI | MM | AJS | |
| 6400 Series/Ultivo | ESI-L Tuning Mix | APCI-L Tuning Mix | APCI-L Tuning Mix | MMI-L Tuning Mix | ESI-L Tuning Mix |
| 6200/6500 Series | ESI-L Tuning Mix* | APCI-L Tuning Mix | APCI-L Tuning Mix | MMI-L Tuning Mix* | ESI-L Tuning Mix* |
Table 2. Agilent Triple Quadrupole, TOF, and Q-TOF tune mix matrix
* May require dilution.
Depending on system model and tune type, the tuning mix may need to be diluted for Q-TOF systems.
Dilute the tune mix when there is an error message that instructs you to do so, or when the tune report shows calibration abundances greater than 750,000 counts, as shown in the following picture.
Figure 1. Calibration abundance for Q-TOF systems
1. First label, 2. Second label, 3. Third label..., etc. (Remove this line if there are no labels).
See the Calibration and Tuning section in the MassHunter Acquisition Online Help for dilution instructions for 6200 TOF and 6500 Series Q TOF systems. This information can be accessed from the Help menu in MassHunter, or by pressing the F1 key in any relevant MassHunter Workstation window or dialog box.
The Agilent Knowledge Portal article also gives detailed instructions on how to dilute the tune mix depending on the source type installed on the system.
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Learn how to effectively operate your Agilent LC/MS System:
LCMS-6100-1101e - Tuning Agilent Single Quadrupole LC/MSD Systems in OpenLab CDS LCMS-6100-2120s - Agilent LC/MSD iQ: How to Build Acquisition Methods Using Auto Acquire and Advanced Acquire in OpenLab CDS LCMS-6400-1120sV2 - Tune and Calibrate Agilent 6400 Series Triple Quadrupole LC/MS with MassHunter Acquisition (Ver. 10.0) LCMS-ULTV-1120s - Tune and Calibrate Agilent Ultivo LC/TQ with MassHunter Acquisition (Ver. 1.1) e-learning courses available from Agilent education |
