Good morning, fellow colleagues. I have started to use MassHunter B.04.00 with my 6520B LC-QTOF. I am having some trouble setting up the diode-array detector on the HPLC from MassHunter. When loading in a HPLC method, the computer responds, Failed to…
Hi Anatoly,
if the vapour pressures of the solvents are comparable it doesn't matter that you inject different solvents and even if they are very different like water vs. any organic solvent, the error is small.
In direct injections and cold-on-column…
Hi, exactly what you said and like what Lance Nelson pointed out,the septum purge flow cannot set on GC 6890 but we can control the septum flow by reduce split vent value or split ratio ,so you can reduce split vent value in method 1 from 197.1 mL/min…
Hello, I've been spending time getting my university's recently donated LCMS up and running, and I've been obtaining good results. This morning, however, the LCMS has been throwing errors every few minutes that's disrupting runs and ability to auto tune…
Hello Nur5ik
Thank you for your question.
Disconnect or loosen the sample probe and rerun the "restriction and pressure decay test".
If you pass the first step, sample prove might be plugged. You need to change sample probe.
If the same step…
Well, I thank everyone for their help. I am learning, and so I set the DIP switches were pushed into 1,2,8 only high. Then restarted. It restarts and then fails. The problem is that the Lab Advisor is running via RS232 and it stops communicating because…
I am using Chemstation to automatically export 7890A and B GC-FID single page reports to PDF at the same time as printing a hard copy report for each injection. If I go into the data analysis software later and reintegrate my peaks, the software doesn…
Aux 2 is typically configured as the transferline heater/sensor. The simplest thing is to vent the MS, turn off the GC, remove the column, slide the GC away from the MS, and then unplug the transferline heater/sensor connector, plug it back together,…
Hi azrael, The FID is not a pressurized detector so there is no pressure test. You may wish to explore our GC Fundamentals series on our website. See Below for a link Fundamentals of Gas Chromatography (GC) - Part 1 | Agilent Regards James
Thanks for the help, What I failed to mention in my poorly worded initial post is that the issue was trying to bring our instrument up in standby. I had vented it, thinking we would store it this way for some time. After having done so, I was informed…
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Can I use reaction volumes larger than 30 μL on the AriaMx?
Resolution
There is no problem with using higher reaction volumes, but the instrument performance can only be...
This Information Applies To: Agilent OpenLab CDS software version 2.3 or higher.
Issue
Chromatogram gridlines can help users to get a better understanding of the scale of the peaks. However, in some cases, the gridlines are not desired, such as...
How to Reset the Agilent GC/MSD Tune File to Default Values
Multiple Results
This Information Applies To: Agilent GC/MS MassHunter Acquisition Software
Issue
If there is a problem with the current tuning parameters and a need to tune from the default parameters, see the following procedure to reset the EI tune file (atune...
This Information Applies To: Agilent OpenLab CDS (version 2.x)
Contents:
In the following procedure, you will find steps to create a multipoint ESTD calibration method with OpenLab CDS 2.X.
Steps to follow:
1. Load the Data
a. From Data...
This Information Applies To: Agilent MassHunter Quantitative Analysis Software
Issue
Chromatographic peak integration is a critical part of accurate and reproducible quantitative data analysis. The integrator type and the integration parameters...
This Information Applies To: Agilent 6200/6400/6500 Series LC/MS Systems
Issue
Instructions on how to fill the calibrant bottle on 6200/6400/6500 Series LC/MS Instruments when calibrant levels are running low.
Background
The ability to autotune...
This Information Applies To: All Agilent 6000 Series LC/MS Systems without a gate valve for ion capillary installation/removal.
Issue:
Installing the Ion Transfer Capillary after cleaning or replacement. For instructions for installing an ion capillary...
This Information Applies To: AriaMx Real-time PCR System
Issue
The saturation level is unique for each optical module after the offset of calibration factor and background calibration. The FAM, ROX, HEX, CY3 & CY5 optical modules will saturate at...
This Information Applies To: Agilent LC Systems
Issue
While it is possible to combine modules from different HPLC series such as 1100, 1200, 1260 or 1290 series to create a functioning LC system, there are some important topics to consider.
...
Fixes for the "RPC Server Unavailable" Error
Multiple Results
This Information Applies To: Agilent OpenLab CDS
Issue
When launching the instrument Acquisition screen/instrument session, there is an "RPC Server Unavailable" error.
Resolution
Accessing the Services App (Windows)
There are two ways to...