Part 1 included many questions and some answers about the maximum abundance that should be detected at the electron multiplier. Another important consideration is how much sample should go into the column.
See Which Electron Ionization Ion Source? Part…
Thank you for taking a moment to review the Community Guidelines. You agree to all of the guidelines posted and linked here when using the Agilent Community. These guidelines are here to help you be a valuable contributor to the community and make sure…
Hi, I have an old 6890 with a jet direct card. We lost power to the instrument and since then it has not been able to connect to the PC. I tried updating the bootp software with the correct mac address and power cycled instrument with the same issue.…
Support and educational resources for the Microplate Centrifuge and Automated Centrifuge Loader .
Automation Solutions Knowledge Base
Microplate-Handling Device Guides
PDF user guides
Support Documents
G5582A Centrifuge Installation Checklist…
-Have you tried a diferent column ? An old column could have retention time issues and peak distortion.. -Have you had any pressure fluctuations during your run ? Try running a Pump leak rate test, to see if the problem is not in the pump-head. -Do a…
On a G1329B autosampler I lose the plastic housing to have access to a valve. Then I forgot to reassemble it and when turn the unit on, the needle went to the top.
Now, I cannot put the plastic housing back, and interferes with the arm.
I…
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Can I use reaction volumes larger than 30 μL on the AriaMx?
Resolution
There is no problem with using higher reaction volumes, but the instrument performance can only be...
This Information Applies To: Agilent OpenLab CDS software version 2.3 or higher.
Issue
Chromatogram gridlines can help users to get a better understanding of the scale of the peaks. However, in some cases, the gridlines are not desired, such as...
How to Reset the Agilent GC/MSD Tune File to Default Values
Multiple Results
This Information Applies To: Agilent GC/MS MassHunter Acquisition Software
Issue
If there is a problem with the current tuning parameters and a need to tune from the default parameters, see the following procedure to reset the EI tune file (atune...
This Information Applies To: Agilent OpenLab CDS (version 2.x)
Contents:
In the following procedure, you will find steps to create a multipoint ESTD calibration method with OpenLab CDS 2.X.
Steps to follow:
1. Load the Data
a. From Data...
This Information Applies To: Agilent MassHunter Quantitative Analysis Software
Issue
Chromatographic peak integration is a critical part of accurate and reproducible quantitative data analysis. The integrator type and the integration parameters...
This Information Applies To: Agilent 6200/6400/6500 Series LC/MS Systems
Issue
Instructions on how to fill the calibrant bottle on 6200/6400/6500 Series LC/MS Instruments when calibrant levels are running low.
Background
The ability to autotune...
This Information Applies To: All Agilent 6000 Series LC/MS Systems without a gate valve for ion capillary installation/removal.
Issue:
Installing the Ion Transfer Capillary after cleaning or replacement. For instructions for installing an ion capillary...
This Information Applies To: AriaMx Real-time PCR System
Issue
The saturation level is unique for each optical module after the offset of calibration factor and background calibration. The FAM, ROX, HEX, CY3 & CY5 optical modules will saturate at...
This Information Applies To: Agilent LC Systems
Issue
While it is possible to combine modules from different HPLC series such as 1100, 1200, 1260 or 1290 series to create a functioning LC system, there are some important topics to consider.
...
Fixes for the "RPC Server Unavailable" Error
Multiple Results
This Information Applies To: Agilent OpenLab CDS
Issue
When launching the instrument Acquisition screen/instrument session, there is an "RPC Server Unavailable" error.
Resolution
Accessing the Services App (Windows)
There are two ways to...