The GC is telling us that the front inlet pressure is 'Not Ready' is there a chance there is a leak in the inlet somewhere after the maintenance was performed?
Can the inlet hold pressure? If it can't hold pressure or does have a leak it will stop the…
Hello cpostigo ,
This error will occur if the batch has not been analyzed and there is no calibration curve available for your targets. The entries do appear to be accepted, and the calculation will be performed once the batch has been fully analyzed…
Hi, DP stands for Drug Product (I need to determine EtOH in a drug product not in a drug substance DS).
I'm loading 3 mL of sample in vial size 20 mL. The concentration of EtOH 4 mg/mL.
Yes of course, three replicates of three separate vials (same…
The Digest of upcoming webinars has been updated as of May 14.
The new Agilent Community Group, GPC/SEC Solutions Group hosted a webinar series in partnership with Dow in April: A Journey through SEC, Light Scattering, and 2D-LC for Macromolecules …
How can I create a method?
To create a method, settings can be selected on the setup page, e.g., wavelength range, averaging time, spectral bandwidth, etc. Some settings are application specific, e.g., kinetic, thermal. Baseline or zero correction can…
This webinar will comprehensively explore transmittance and reflectance measurements for both transparent and non-transparent crystal and thin film materials. Through detailed studies, it will be demonstrated how to accurately estimate the effects of…
Hello,
I would like to have some feedback from anyone that has the Agilent 6475. Is it a versatile instrument? Does it provide a level of sensitivity that allows for excellent quantitation? I am interested in a system that will allow me to analyze a…
The Headspace autosampler driver is not installed by default on the client computers. It is classed as a special injector, which it is.
On the original OpenLab installation media, a USB stick, navigate to the following directory, on the client PC…
This Information Applies To: Agilent OpenLab CDS (version 2.x)
Contents:
In the following procedure, you will find steps to create a multipoint ESTD calibration method with OpenLab CDS 2.X.
Steps to follow:
1. Load the Data
a. From Data...
This Information Applies To: Agilent MassHunter Quantitative Analysis Software
Issue
Chromatographic peak integration is a critical part of accurate and reproducible quantitative data analysis. The integrator type and the integration parameters...
This Information Applies To: Agilent 6200/6400/6500 Series LC/MS Systems
Issue
Instructions on how to fill the calibrant bottle on 6200/6400/6500 Series LC/MS Instruments when calibrant levels are running low.
Background
The ability to autotune...
This Information Applies To: All Agilent 6000 Series LC/MS Systems without a gate valve for ion capillary installation/removal.
Issue:
Installing the Ion Transfer Capillary after cleaning or replacement. For instructions for installing an ion capillary...
This Information Applies To: AriaMx Real-time PCR System
Issue
The saturation level is unique for each optical module after the offset of calibration factor and background calibration. The FAM, ROX, HEX, CY3 & CY5 optical modules will saturate at...
This Information Applies To: Agilent LC Systems
Issue
While it is possible to combine modules from different HPLC series such as 1100, 1200, 1260 or 1290 series to create a functioning LC system, there are some important topics to consider.
...
Fixes for the "RPC Server Unavailable" Error
Multiple Results
This Information Applies To: Agilent OpenLab CDS
Issue
When launching the instrument Acquisition screen/instrument session, there is an "RPC Server Unavailable" error.
Resolution
Accessing the Services App (Windows)
There are two ways to...
This Information Applies To: Agilent LC Pumps
Issue
If a user suspects that the gradient composition isn’t accurate and causes poor chromatography such as retention time shifts, late, early, no peaks or merging peaks. This article provides a quick...
This Information Applies To: Agilent 1290 Infinity (G4226A) and Agilent 1260 Infinity (G1367E) HiP Autosampler
Issue
Replacing the Needle Seat of the Autosampler is required when:
The needle seat is visibly damaged.
Leaks or blockages in the...
This Information Applies To: Agilent OpenLab CDS
Issue
This article discusses different types of bracketing modes and how to set up a bracketed calibration in Data Analysis for OpenLab CDS 2.x
Background
Occasionally, the instrument conditions...