Hi, We observed an error during autotune of QQQ GCMS as "Peak width gain failed", When it was occurred and what is the reason for the repeat error, Autotune was not completed due to " Peak width gain failed".
Please suggest the recommendations and possibility…
Want to quantify or at least qualify trifluoroacetic acid (tfa) on our column, which is almost exclusively used for quantifying propionic acid, with acetic acid as the internal standard. Both dissolved in acetonitrile. It seems like tfa would work, but…
Hi Everyone, I'm operating our Cary 3500 and I am helping with an assay being put together based on competitive binding. Because of the competitive binding, the primary and reliable peak *decreases* in size rather than increasing as the concentration…
jhagel is correct, do not change PE (Yellow) to PTFE (black) seals in round headed pumps (1290 Infinity and Infinity II as well as G7014C). Both seal types have benefits and downsides: While the black seals are generally better when using them with reverse…
I have a 5977B MSD that is experiencing a vacuum failure after a scheduled shutdown for renovation work in our facility. Work was completed and now we are restarting the system. At first we though it was the roughing pump, but we have bypassed the remote…
Hello,
I have a G6545 QTOF / 1260 HPLC system that came with an HP Z440 workstation with Windows 10 as part of the purchase from Agilent. The workstation is ~ 3 years old. The instrument was running normally over the weekend -- when I came back, the…
Also, please make sure that a default printer is configured in Windows. If no default printer is configured, the following problems may occur: - Printing of a report preview will fail. - the 'Copy To Clipboard' menu will have an error - the 'Custom Report…
Im running on an old GCMS with headspace sampler. Its been failing during runs recently. The logbook shows that the oven is not ready at run time for each sample. After about 10 samples it just stops collecting data. The headspace continues to cook all…
Thank you for your question. Unusual high low voltage error is a common instrument and accessory maintenance related and not instrument hardware related issue. Typically, when you see that error, under logbook of your run, you will see chip map. Please…
This Information Applies To: Agilent G1312X and G7112X binary pumps.
Issue:
A blockage before the pressure sensor, e.g. in a pump head, will not give a high pressure or overpressure system error. Instead, the pump may show one of various error messages...
This Information Applies To: Agilent 1100, 1200, 1260 LC Pumps.
Issue:
The following procedure allows the cleaning of residue from the pump's check valves, often caused by polymerization of aged acetonitrile or salt-based solutions that may prevent...
This Information Applies To: Agilent LC Systems
Issue
After analyzing dirty or concentrated samples, some sample compounds may retain in the column and other parts of the HPLC system. If the column re-equilibration after each run is not enough to...
This Information Applies To: Agilent G7167A/B Multisampler.
Issue:
Required when:
The needle is visibly damaged.
Leaks or blockages in the vialsampler are observed, especially between the needle and needle seat.
The limit for the needle...
This Information Applies To: Agilent Autosampler modules G1367A, G1367B, G1367C, and G1367D.
Issue:
The removal and installation of the needle assembly in these older autosampler modules can be tricky and may require patience and the correct technique...
This Information Applies To: All standard analytical scale Agilent 1100/1200/1260/1290 Infinity / Infinity II LC system configurations: Single Pump (Isocratic, Quaternary, or Binary) with Manual Purge Valve. Does not apply to systems with Agilent G7167A...
This Information Applies To: All Agilent LC modules (except hosted modules).
Issue:
When a module does not initialize correctly or it is not recognized by the software, it is recommended to call our Remote Support Representatives to troubleshoot...
This Information Applies To: All Agilent LC pump modules with MCGV installed (quaternary pumps)
Issue:
Shifting retention times: The composition of the mobile phase in liquid chromatography directly influences retention times. If any of the channels...
This Information Applies To: All Agilent liquid chromatography instruments, Lab Advisor
Issue:
The error message 'Could not open channel RD' pops up when performing diagnostic tests in Lab Advisor. Retrying the test is not helping.
Resolution...
This Information Applies To: Agilent Lab Advisor Software for any Agilent LC System.
Issue:
To successfully troubleshoot an Agilent LC system or module, the System Report is an essential resource. Agilent Support representatives may ask you to provide...