This Information Applies To: All Agilent LC pump modules with MCGV installed (quaternary pumps)
Shifting retention times: The composition of the mobile phase in liquid chromatography directly influences retention times. If any of the channels of the MCGV are partially or fully blocked, the gradient produced will be different from the analytical method.
Clogging of the MCGV may occur when the system is not rinsed properly or stored incorrectly.
If the following steps don’t solve the shifting retention time issue, the MCGV may be damaged or leaking and may not be working correctly. This might require a replacement of the MCGV assembly.
Steps to follow:
Learn how to effectively operate your Agilent Quaternary Pump:
Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps user manual on Agilent.com