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Articles Forced Cold Start Procedure
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  • Created :  10 Nov 2022
  • Modified :  2 Aug 2023
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Analytical LC Systems Preparative LC Systems
  • Component :  LC Column Compartments LC Detectors LC Fraction Collectors LC Injectors and Sample Thermostats LC Pumps LC Valves
  • Product Name :  1100 Series LC System 1120 Compact LC 1200 Series LC System 1220 infinity II LC System 1260 Infinity II Bio-Inert LC System 1260 Infinity II LC System 1260 Infinity II Prime Bio LC System 1260 Infinity LC System 1260 Infinity II Prime LC System 1290 Infinity II Bio LC System 1290 Infinity II LC System 1290 Infinity LC System
  • Task :  Operation Troubleshooting
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Forced Cold Start Procedure

Answer

This Information Applies To: All Agilent LC modules (except hosted modules).


Issue:

When a module does not initialize correctly or it is not recognized by the software, it is recommended to call our Remote Support Representatives to troubleshoot the issue. You may be asked to follow the cold start procedure to recover the correct functionality of the module. A forced cold start can be used to bring the module into a defined mode with default parameter settings.

 


Before starting:

Save your methods and data before executing a forced cold start. A forced cold start erases all methods and data stored in the nonvolatile memory of the module, i.e a non saved state.
Exceptions are calibration settings, diagnosis, and repair logbooks, which will not, be erased.

 

Do not use a pencil or a fragile tool to move the switches to avoid breaking it inside the module. We recommend you to use a screwdriver or a pen.

 

Steps to follow:

  1. Switch off the modules.
  2. Disconnect the cables that connect the troubled module to the others.
  3. Take note of the current configuration of the switches that are on the back of the module inside the CONFIG slot (Figure 1). This step is important, because after the procedure you will have to move the switches back to that original configuration. Your module might have 2, 6 or 8 switches depending on the model. All Infinity II Series modules have a 6-switch configuration, except the Hosted modules that use a 2-switch configuration (Multicolumn Thermostat, Flexible Cube, UIB II, and 1290 Infinity Valve Drive). The 8-switch configuration is for 1100 and 1200 Series instruments.
  4. Change the switches configuration according to Table 1.
  5. Switch on the instrument and wait 3 minutes until the initialization is completed.
  6. Switch off the instrument.
  7. Place the switches in their original position.
  8. Reconnect all the cables and switch on all the modules in the stack. 

 

Figure 1. Location of Configuration Switch (CONFIG) and different type of Switches of HPLC modules.

 

 

 

 Table 1: Switches' configuration for a forced cold start. 1 means up, 0 means down.  

2 switches 1 0
6 switches 1 0 0 0 0 1
8 switches 1 1 0 0 0 0 0 1

 

 

Special considerations: 

 

The instrument will always revert to the default state after a cold start. It is especially important for plate types and the metering head size in autosamplers. For example, the 900 µL analytical head upgrade will not be recognized. It must be set again in the software (the same as during initial installation). The sampler will default to the standard 100 µL head.

 

 Tip  Learn more on how to operate your Agilent LC System:
LC user manual library on Agilent.com
 
 
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