Hi, Using one and the same column for different applications is possible, as long both solvents are permitted (column user manual e.g.).
The column should always be flushed very slowly from solvent A to B (0.1 mL/min). At least 5 times the column volume…
Hi Marty,
Thanks for taking the time to respond. I saved the data to a file and then printed the file and it was properly formatted. I think that there is an issue with printing to paper from the method. Since this is a long unsupported CS revision…
Hi Abbey
Thanks for the info!
So far we have only been running this on various forms of olive oil. But the waves in the baseline remain regardless of MOAH contant. I have run samples that were analyzed by outside labs (with COA) that contain 0ppm…
From a quick google search, it appears that hydroquinone may be detectable through LC/MS (ESI) in both positive and negative mode. It forms a protonated molecule ([M+H]+) at m/z 111 in positive mode and two ions at m/z 108 and 109 in negative mode.
…
奔跑的小短腿 , While an LC/MS system may operate in both positive and negative ion polarity modes, it will switch between the two polarities based on the user input in the acquisition software. It is not possible to acquire in both polarities simultaneously…
Hi Vincenzo,
Thank you for your answer. We have the CFR environment. WinUV 5.1.3.1042. (1042 indicates Part11).
I’ve looked for method files for the following apps; Advanced Reads which are .MAB, Concentration .MCN, Scan .MSW, and Simple Reads .MSR…
This Information Applies To: Agilent G1312X and G7112X binary pumps.
Issue:
A blockage before the pressure sensor, e.g. in a pump head, will not give a high pressure or overpressure system error. Instead, the pump may show one of various error messages...
This Information Applies To: Agilent 1100, 1200, 1260 LC Pumps.
Issue:
The following procedure allows the cleaning of residue from the pump's check valves, often caused by polymerization of aged acetonitrile or salt-based solutions that may prevent...
This Information Applies To: Agilent LC Systems
Issue
After analyzing dirty or concentrated samples, some sample compounds may retain in the column and other parts of the HPLC system. If the column re-equilibration after each run is not enough to...
This Information Applies To: Agilent G7167A/B Multisampler.
Issue:
Required when:
The needle is visibly damaged.
Leaks or blockages in the vialsampler are observed, especially between the needle and needle seat.
The limit for the needle...
This Information Applies To: Agilent Autosampler modules G1367A, G1367B, G1367C, and G1367D.
Issue:
The removal and installation of the needle assembly in these older autosampler modules can be tricky and may require patience and the correct technique...
This Information Applies To: All standard analytical scale Agilent 1100/1200/1260/1290 Infinity / Infinity II LC system configurations: Single Pump (Isocratic, Quaternary, or Binary) with Manual Purge Valve. Does not apply to systems with Agilent G7167A...
This Information Applies To: All Agilent LC modules (except hosted modules).
Issue:
When a module does not initialize correctly or it is not recognized by the software, it is recommended to call our Remote Support Representatives to troubleshoot...
This Information Applies To: All Agilent LC pump modules with MCGV installed (quaternary pumps)
Issue:
Shifting retention times: The composition of the mobile phase in liquid chromatography directly influences retention times. If any of the channels...
This Information Applies To: All Agilent liquid chromatography instruments, Lab Advisor
Issue:
The error message 'Could not open channel RD' pops up when performing diagnostic tests in Lab Advisor. Retrying the test is not helping.
Resolution...
This Information Applies To: Agilent Lab Advisor Software for any Agilent LC System.
Issue:
To successfully troubleshoot an Agilent LC system or module, the System Report is an essential resource. Agilent Support representatives may ask you to provide...