The Agilent Cary 3500 Flexible UV-Vis system is a versatile instrument used for analyzing a privacy screen's light transmission properties at various angles and light conditions. It boasts a spacious sample compartment suitable for different accessories…
One of the most challenging tasks for vacuum system designers is to create ultra-high and extreme-high vacuum pressures. Pumps capable of generating UHV and XHV pressure include Sputter Ion Pumps, Titanium Sublimation Pumps and Non-Evaporative Getters…
A system with poorly designed conductance can have drastic consequences on the effective pumping speed: the cornerstone of this tutorial is to emphasize the need to design a system that does not have negative effects on the performance of a high or ultrahigh…
Thermal gauges, out of all the available gauge technologies, offer excellent value for measuring rough vacuum pressure, but even UHV and XHV systems can benefit from them. All thermal gauges operate by heating a filament and exposing it to the vacuum…
When detecting leaks, it is important to distinguish between virtual and real ones. Examples of virtual leaks can be: gas desorption, diffusion, trapped volume, and back streaming. Leak-up or rate-of-rise tests can indicate whether a vacuum system's base…
Ionization gauges convert residual molecules to positively charged ions, and then attract them to a negatively charged collector where they create a measurable current. Modifications to high vacuum ionization gauges can optimize their performance at a…
No one gauge technology will provide pressure measurement throughout the range of vacuum. Combining vacuum sensors can produce a gauge capable of measuring pressure better than with any single technology. After having highlighted the properties of individual…
When choosing a vacuum gauge, it is possible to adopt high vacuum ionization gauges or sputter ion current. After describing the basic operating principle of the Sputter ion pump, this video shows how to measure UHV pressure with Agilent SIPs.
This Information Applies To: Agilent 6820, 6890, 7820A GC systems with a Split/Splitless (SSL) inlet
Issue
Non-volatile sample matrix components will accumulate in the inlet liner and impact on analytical performance. The inlet liner should be changed...
This Information Applies To: Agilent headspace samplers (HS)
Issue
GC cycle time needs to be calculated correctly. Too short a GC cycle time will reduce sample quality and possibly stop the analysis prematurely. Too long GC cycle time can cause...
This Information Applies To: Agilent 7820A GC
Issue
7820A GC is designed with simplified keypad that comes with five buttons. They are Stop, Prep Run , Start, Up and Down arrow buttons. Changing the IP address requires a different method compared...
This Information Applies To: Agilent Single Needle Autosamplers
Background
Sometimes the user may observe liquid flowing from Port four on the autosampler injection valve. During an injection sequence, it is normal to have liquid from port four...
This Information Applies To: Agilent 4200, 4150, 2200 TapeStation systems
Issue
Can I run an assay using the ladder or sample buffer from a different assay if I have run out of the appropriate assay ladder or sample buffer? Is it possible to run...
This Information Applies To: Agilent ICP-MS 7700, 7800, 7850, 7900, 8800, and 8900 systems
Issue
Peristaltic pump tubing that has lost its elasticity will not deliver the sample to the nebulizer correctly. When the pump tubing appears limp or flattened...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx touch screen displays error code 90 after the Instrument Health Check (IHC), which happens when the AriaMx is switched on, or after running the onboard diagnostic tests...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx optical modules
Issue
AriaMx users can change which AriaMx optical modules are installed to upgrade the instrument capabilities or to troubleshoot.
Items Required
Any...
This Information Applies To: AriaMx real-time PCR system
Issue
Error 43 is displayed on the AriaMx touch screen. Error 43 is a voltage error and it normally indicates that the cartridge LED voltage or motor voltage being detected is abnormal. Sometimes...
This Information Applies To: Agilent 2100 Bioanalyzer system
Issue
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Background
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real...