Hi all, I'll contribute to this topic with a few comments. I'm in no way a GC expert but I have been working with a GC-MS, basically on my own, for about a year now and I have been trying to gain knowledge on how to best operate the machine. Please correct…
Hi Ron, cheers for that. I have made a note of that method flow, thanks, but it's looking like it may be a touch moot; I cannot find the discs anywhere. I have sent an email to our IT team to see if they have them, but otherwise I'm at a bit of a loss…
I am having trouble separating methyl methacrylate (MMA) from another peak. The interfering compound, appearing in the front, is identified by library as 3,3-dimethyl-2-butanone and it is inherent to the sample. MMA appears as the split/tailing peak.…
Hi, we are currently unable to get Helium. We'll run our Helium gas inventory in a few weeks. Between other applications for our facility, it is used as a carrier gas on our GCs for solvent analysis (validated method). I'm copying our method below. Would…
Resurrected a 6890 which has not been used for a while. When the chemstation is turned on, it gives message of "Limits fail: liner(injection)". Pulled out and cleaned/replaced microseal (fine), glass liner with o-ring (no physical samage) and golden seal…
Hi Nick- Thanks for that information! I'm not sure if that's the cause here. It was 8 million when it was installed in December 2015, in August 2016 it was 9 million, March 2017 it was 8 million, April 2017 was 7 million, then August 2017 it was only…
Thanks ryoboyle and dheyaaldeen . I renamed the 4 files (config.reg, ChemStationPerInstrumentSettings.config, instrument.config, RapidControl.InstrumentConfig.xml). I do not have a user.mac file in the CORE folder. The program opened once and I reconnected…
Hi all, The GC in my lab is Agilent 7820A that my supervisor bought it 2-3 years ago. The configuration of GC is as follows: - One detector FID - One inlet Split/Splitless - One line for gas sample and One gas sampling valve. However, I am not sure there…
megaflorch - this was fairly old topic and I wasn't sure if you completed your standards/sample prep. I use a similar technique to get my analytes within a better working linear range with respect to my GC-FID. Your logic is good.... here is my standard…
I have a simple syntax question (I think). I am trying to get the statement below to work, but am having a problem with the syntax at the Select statement. Any advice would be great: I tried this and it is failing at " x. GetCustomField ( "Sample Weight…
This Information Applies To: 4200, 4150 and 2200 TapeStation, TapeStation analysis software A02.02 or higher.
Issue:
When using the Agilent 4200, 4150 and 2200 TapeStation systems, the DV 200 of FFPE RNA samples can be calculated within the TapeStation...
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer, ProSize Data Analysis Software
Issue
How to export a ladder as a size calibration file and import it into a different run in ProSize.
Background
...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
It is possible to set up the 2100 Expert software so that the files are automatically exported each time a chip run is complete.
Steps to Follow
To turn...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software
Issue:
How to identify and troubleshoot late migration of the ladder, marker, and sample peaks in data generated on the Agilent 2100 Bioanalyzer system.
Background
...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
If communication between the 2100 Bioanalyzer and the PC cannot be established, or if an intermittent communication loss occurs, follow the instructions in...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
Instructions on how to import from or export data into LIMS software.
Steps to Follow
Data import
When importing sample names and sample comments from...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software version B.02.08 or later
Issue
When printing a report to PDF or HTML with the 2100 Expert software, the error "Failed to Generate Report" appears.
Background
This error...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software
Issue
Resetting upper or lower markers from their automatically assigned settings in the Agilent 2100 Expert software.
Background
The Agilent 2100 Expert software...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert Software
Issue
Samples with a broad signal such as NGS smears should be analyzed by setting up a region. Analyzing a region is also termed smear analysis.
Steps to Follow...