It really does not matter whether you do a wavelength calibration axial or radial, because the wavelengths hitting the detector are the same. The relative intensities will be different though, so the specific wavecal worksheet has specific threshold intensities…
It's going to be difficult to determine what is going on without seeing your batches. I would recommend that you contact tech support or your local Agilent applications chemist and send them your batches so they can help you.
Some COVID-19 vaccines are using lipid-based nanoparticles to act as drug delivery systems for effective on-target payload delivery. Just like any finished product components, lipid nanoparticle components—like pegylated lipids, cholesterol, ionizable…
Hello,
As Cole said you need to be selecting the correct sample types in you sequence to use this. If you are doing that, the filter would be Sample_Type <> 1 to remove the calibration standards or Sample_Type = 3 for just samples if you have other…
Hello,
When you say results does that mean raw data or reports? For the raw data you can control the storage location using projects and then subfolders under the project. If you want the reports to go to a central share in a specific folder that…
Thank you Sabbas for your question.
The Tutorials and Help in Cary WinUV 5.3 is also relevant for many questions, applications and workflows a Cary WinUV 5.1.3 user might have. However, some sections might be specific and different for Cary WinUV 5…
This Information Applies To: Agilent HES source, Agilent 5977 Series GC/MSD
Issue:
During normal operation of the GC/MSD, the source will require cleaning. It is time to clean the source when:
There is a loss in analyte response not improving...
This Information Applies To: Agilent 5977 and 5975 Series GC/MSD systems
Issue
The autotune fails and reports the error Tune action stopped: Gain and offset = 0 and peaks are too narrow!
Resolution
The MSD autotune program is run after routine...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
Condition new or cleaned ICP-MS skimmer cones and sampler cones by aspirating a Conditioning Solution before use.
Background
Drift up can be a sign of...
Turn On the GC/MS Interface and Oven Using OpenLab CDS
Multiple Results
This Information Applies To: Agilent GC/MS instruments controlled by OpenLab CDS software
Issue
Turning on the GC/MS interface heater and Oven using OpenLab CDS software.
Steps to Follow
From the Home tab, click Method > Instrument Setup...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900)
Issue
The signal monitor function in MassHunter can help with troubleshooting low sensitivity, carry over, contamination, or high background. It can also be used to optimize...
This Information Applies To: Agilent UV detectors (Variable Wavelength Detectors, Multiple Wavelength Detectors, and Diode Array Detectors)
Issue
Checking accumulated hours on the UV lamp.
Steps to Follow
There are four ways to check the accumulated...
This Information Applies To: Agilent 7700/7800/7850/7900/8800/8900 ICP-MS
Issue:
Poor internal standard (ISTD) mixing can cause high relative standard deviation (RSD).
The recommendations in this article are applicable if:
It takes a long...
This Information Applies To: Parallel capillary electrophoresis platforms, 5200/5300/5400 Fragment Analyzer, ZAG DNA Analyzer, Femto Pulse, Oligo Pro II
Issue:
Disposing used capillary arrays
Resolution:
Arrays can be discarded in regular...
This Information Applies To: 5200, 5300 and 5400 Fragment Analyzer, ProSize software, DNF-471 RNA kit, DNF-472 HS RNA kit
Issue:
The DV 200 score is a quality score for evaluating quality of RNA derived from formalin-fixed paraffin-embedded (FFPE...
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, and ZAG DNA Analyzer
Issue:
Dirt or dust on the capillary array window can cause baseline shifts or elevation of the baseline. Figure 1 shows an example electropherogram...